Analysis of Microsatellite Distribution Characteristics in the Genome of Nemichthys scolopaceus
Inthis study,the MISA tool was used to search microsatellite sites from 57.93 Gb genomic se-quencing data of Nemichthys scolopaceus for the first time.Microsatellite distribution characteristics were ana-lyzed and the results showed that 1 113 480 loci with the length of 14 044 174 bp were obtained,which were randomly dispersed in 813 780 scaffolds.The occurrence frequency,appearance frequency and relative abun-dance were 11.73%,16.05%and 591.51/Mb,respectively.Among the microsatellites with the repeat unit length of 1-6 bp,dinucleotide repeats accounted for the largest proportion(558 274,50.14%),the highest ap-pearance frequency(8.05%)and the greatest relative abundance(296.57/Mb).A total of 1 484 microsatellite repeat motifs were detected,in which the hexanucleotide repeat motifs were the most abundant(603),and then followed by pentanucleotide(575),tetranucleotide(230),trinucleotide(60),dinucleotide(12)and mononucleotide(4)in turn.Their preponderant repeat motifs were A(178 791,47.83%),CA(158 182,28.33%),AAT(13 790,13.21%),CACG(4 584,6.79%),AATTG(166,3.71%)and CACACG(439,8.66%),respectively.Research da-ta involving the number,distribution and abundance of different microsatellites in the genome of N.scolopaceus will provide basis for the development of high-quality microsatellite markers of this species in the future,and also lay the foundation for researches on germplasm resources of several related fishes.
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