Analysis of Mitochondrial DNA Copy Number Difference in Diverse Tissues of Chiloscyllium plagiosum
Mitochondrial DNA(mtDNA)copy number varies in different tissues and organs,and is closely related to the occurrence of many important diseases.The stability of mtDNA copy number is essential for maintaining mitochondrial function and cell metabolism.Therefore,accurate monitoring mtDNA copy number is important for biological and biomedical research.However,the current commonly used analytical methods can only perform relative quantitative analysis of mtDNA copy number,and cannot accurately quantify mtDNA con-tent.In this study,a method for quantitative evaluation of mtDNA copy number was established by using high-throughput sequencing data and bioinformatics analysis software NOVOPlasty,and this method was used to quantitatively compare and analyze mtDNA copy number in muscle and blood tissue samples of Chiloscylli-um plagiosum.The results showed that the mtDNA content of blood tissue was evidently less than that of mus-cle tissue.The mean mtDNA copy number of muscle tissue at 1×whole genome sequencing depth was 339,while that of blood tissue was about 15,suggesting that the mtDNA content of muscle cells was about 22 times higher than that of blood cells.The results showed that the mtDNA content in the muscle and blood of C.pla-giosum had obvious tissue specificity,and the feasibility of the mtDNA copy number quantitative analysis method established in this study was preliminarily verified.This study can provide reference materials for biomedical and ecophysiological studies related to mtDNA content.
mitochondrial DNA copy numberChiloscyllium plagiosumquantitative analysistissue specificityhigh-throughput sequencing
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