Objective To detect the role of low-density lipoprotein receptor-related protein 1(LRP1)in malignant tumors of the digestive system.Methods The cancer genome atlas(TCGA)database was used to analyze the expression of LRP1 in tumors.R software(version 3.6.3)was used to analyze the relationship in survival and prognosis between LRP1 and malignant tumors of the digestive system.The paracancerous tissues and tumor tissues of patients with gastric cancer,liver cancer and pancreatic cancer were collected for HE staining to observe the expression of LRP1.LRP1 expression were reduced by using RNA interfere lentivirus in malignant tumor cells of the digestive system,and malignant tumor cells of the digestive system biological characteristics were observed after LRP1 knocked down.Detected changes in protein expression of malignant tumor cells in the digestive system after LRP1 knockdown through Western Blot,explored the mechanisms by which LRP1 influences the proliferation,invasion,and migration of malignant tumor cells in the digestive system.Results Pan-cancer analysis revealed LRP1 was highly expressed in gastric cancer,colorectal cancer,pancreatic cancer and liver cancer tissues.Bioinformatics analysis showed that high expression of LRP1 was closely related to the short survival time and poor prognosis of malignant tumors of the digestive system.At the tissue level,LRP1 was highly expressed in gastric cancer,liver cancer and pancreatic cancer compared with adjacent normal tissues,which was consistent with the results of bioinformatics analysis.The results of the protein levels were shown that LRP1 was highly expressed in HGC-27 cell,HepG2 cell,and BxPC-3 cell.Results showed that reduced LRP1 expression suppressed the proliferation,invasion,and migration of malignant tumor cells in the digestive system.Conclusion Reduce LRP1 expression inhibited the proliferation,invasion,and migration of malignant tumor cells in the digestive system,suggesting that LRP1 may be a new target for the treatment of malignant tumors in the digestive system.
Malignant tumors of the digestive systemLRP1Tumor invasionPrognosis
万方数据
维普
