Objective To analyze the drug resistance of Enterobacter cloacae and explore the situation of aminoglycoside-related drug resistance genes.Methods Six aminoglycoside-modifying enzymes(AMEs)genes,two 16SrRNA methylase genes and Class I integron genes(intl)of these isolates were determined by PCR,and the correlation between these genes and the drug resistance of aminoglycoside was analyzed.Results Besides high resistance to first-generation cephalosporin and individual β-lactamase antibiotics,the Enterobacter cloacae isolates showed relatively well susceptibility to all other tested antibiotics.Among the nine aminoglycoside resistant isolates,five AMEs genes were detected,with the aac(6')-Ib(66.67%)being the most prevalent,armA and intl were detected with a positive rate of 44.44%and 77.78%respectively,while no aac3-Ⅲ was detected.The relatedness of resistance phenotype and genotype in Enterobacter cloacae was 84.24%.Conclusion The prevalence of aminoglycoside resistance is highly related to the genetic contents of AMEs in Enterobacter cloacae.Though the antibiotic resistance of Enterobacter cloacae strains in our hospital represents well susceptibility towards current antibiotics in use,rational application of antibiotics should still be strengthened in clinic.
维普
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