Objective To study the accumulation,adhesion and biofilm formation characteristics of Fusobacterium nucleatum(F.nucleatum)on the surface of commonly used oral materials(titanium,resin denture,polystyrene),and the effect of sugar(sucrose,lactose,xylitol)exposure on it.To reveal the pathogenicity of F.nucleatum in different oral conditions.Methods F.nucleatum was incubated anaerobically on different carrier surfaces and exposed to different sugars for 2 h,4 h,6 h,8 h(adhesion stage),12 h,24 h,48 h,72 h,96 h(biofilm stage).The biomass of F.nucleatum at different time points was detected by crystal violet staining method,and the adhesion rate was calculated.The morphology of F.nucleatum was characterized by field emission scanning electron microscopy.F.nucleatum was incubated anaerobically on the surface of different materials(titanium,resin denture,polystyrene)and in the medium containing different sugars(sucrose,lactose,xylitol)for 48h to form a biofilm.The absorbance value of the supernatant of the biofilm was measured by standing after the bacteria were suspended.The self-aggregation rate was calculated.Results The autoaggregation rates of F.nucleatum on 0.1 m,0.5 m,1.5 m,3 m,4.0 m titanium,polystyrene and resin denture surfaces were 36.14％,53.76％,46.18％,45.08％,44.83％,50.22％,respectively.The autoaggregation rates of F.nucleatum in sucrose,lactose and xylitol were 60.58％,43.36％and 36.50％,respectively.F.nucleatum reached the maximum adhesion rate of 98％,180％,177％,40％at 6 hours on 0.1 m,0.5 m,1.5 m,3 m,titanium disks and resin denture surfaces,respectively.The adhesion rates on 4m titanium sheet and polystyrene surface for 8h were 108％and 548％,respectively.The maximum adhesion rates of sucrose,lactose and xylitol were 370％,196％,144％and 97％,respectively.F.nucleatum reached the maximum biomass of biofilm at 48h and biofilm matured,but biofilm biomass decreased at 72 h and 96 h and biofilm declined.Conclusion F.nucleatum has good ability to aggregate,adhere and form biofilms on different vectors and at different sugar exposures.
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