Objective To detect the effects of different concentrations of itraconazole on apoptosis of human hemangioma endothelial cells(HemECs),and provide basis for the study of the mechanism of itraconazole in the treatment of hemangioma.Methods HemECs cultured in vitro were interfered with different concentrations(30 μg/mL,40μg/mL,50μg/mL)of itraconazole(12 h,24 h).The morphological changes of HemECs were observed by inverted microscope,the cell viability and inhibition rate were detected by CCK-8 method,and the apoptosis rate of HemECs induced by itraconazole was detected by flow cytometry.Results Itraconazole had little effect on HemECs when the concentration of itraconazole was 5 μ g/mL,10μ g/mL and 20 μ g/mL.When the concentration of itraconazole was ≥30 μ g/mL,the effect began to be significant gradually,and there was a positive correlation between itraconazole concentration and apoptosis,and the inhibition rate of each concentration was significantly different from that of the blank group(P＜0.05).The intervention time of itraconazole was also positively correlated with the inhibition of cell proliferation and apoptosis of HemECs.Conclusion Itraconazole can inhibit the growth of HemECs.The higher the concentration,the longer the intervention time,the more significant the inhibitory effect of HemECs on cell proliferation and the promotion of apoptosis.When the concentration is ≥ 30 μ g/mL,the effect is gradually significant,which is positively correlated with apoptosis and inhibition of cell proliferation,and 24 h is the appropriate intervention time.
万方数据
维普
