Objective To evaluate the safety,feasibility and clinical efficacy of flexible ureteroscopy combined with flexible vacuum aspiration ureteral access sheath lithotripsy for renal stones larger than 2 cm.Methods The clinical data of 68 patients who underwent flexible ureterscope combined with flexible vacuum aspiration ureteral access sheath to treat renal stones with a size of larger than 2cm from Nov 2021 to May 2023 were retrospectively analyzed.The stone free rate and complications were evaluated 4 weeks after the operation.Results The renal stones size was 2.1~3.7 cm,with an average of(2.56±0.55)cm.CT value of stones was 638~1,485 Hu,with an average of(929.2±286.9)Hu.The success rate of the ureteroscopic insertion was 100%.The operation time was 50~170 min,with an average of(95.4±27.9)min.The total postoperative stone free rate was 88.24%,including 93.88%in the 2~3 cm group and 73.68%in the ≥3cm group.However,the total complication rate was 2.94%,including 2.04%in the 2~3 cm group and 5.26%in the ≥3 cm group.Post-operative high fever occurred in two cases,which improved after anti-infection treatment.No complications such as massive hemorrhage and organ injury occurred in all cases.Conclusion The flexible ureterscopy with flexible vacuum aspiration ureteral access sheath lithotripsy is safe,feasible,minimal invasive and effective in the treatment of renal stones larger than 2 cm.