Objective To obtain the differentially expressed genes related to obstructive sleep apnea Hypopnea syndrome based on GEO database through bioinformatics technology,to explore its molecular mechanism,and to make predictions on traditional Chinese medicine,in order to provide theoretical basis and new ideas for the integrated diagnosis and treatment of obstructive sleep apnea Hypopnea syndrome.Methods The chip dataset GSE135917 was retrieved by using the keyword"Obstructive sleep apnea homodyne"in the GEO database.GEO2R online analysis was used to screen differentially expressed genes,and the Excel was used to organize and analyze the data to obtain significantly differentially expressed genes.GO and KEGG enrichment analysis,protein interaction(PPI)network construction,and visualization analysis were conducted to obtain the associated signaling pathways and core genes.Finally,the core gene mapping was performed through the Coreminemedical database to obtain disease-related traditional Chinese medicine.Results This study showed a total of 105 differentially expressed genes related to OSAHS.GO enrichment analysis showed that the biological processes of differential genes were mainly concentrated in the reactions to Lipopolysaccharide,glucocorticoid,bacteria derived molecules,Corticosteroid,etc.The cell components were mainly enriched in low-density lipoprotein particles,endocytosis vesicle lumens,High-density lipoprotein particles,lysosome lumens,etc.The main molecular functions are RNA polymerase Ⅱ specific DNA binding transcription factor binding,growth factor activity,DNA transcription factor binding,and Nuclear receptor binding.KEGG enrichment analysis yielded a total of 10 signal pathways,including IL-17 signal pathway,TNF signal pathway,Kaposi's sarcoma associated herpesvirus infection pathway,pathogenic Escherichia coli infection pathway,etc.A total of 12 core genes(IL-6,FOS,F OSB,NR4A1,ATF3,DUSP1,EGR1,JUN,ZFP36,PTGS2,CXCL2,SOCS3)were obtained through protein interaction network construction and visualization analysis.A total of 89 Chinese herbal medicines with potential therapeutic effects were obtained through core gene mapping,with the main effects being tonifying deficiency,clearing heat,promoting blood circulation and resolving stasis,resolving phlegm,cough,and asthma,and expelling wind and dampness.Conclusion The expression of core genes such as IL-6,FOS,and signaling pathways such as IL-17,TNF may play a key role in the course of OSAHS.Some traditional Chinese medicines for clearing heat,promoting blood circulation,removing blood stasis,tonifying deficiency,resolving phlegm,and relieving cough can Targeted therapy OSAHS,with potential therapeutic effects and good application prospects.Bioinformatics analysis can obtain multiple targets for OSAHS treatment,which is of great significance for its precise treatment and integrated traditional Chinese and western medicine treatment.
Obstructive sleep apnea hypopnea syndromeBioinformaticsGEO databaseTraditional Chinese medicine prediction
NETL
NSTL
万方数据
