Objective To investigate the mechanism of Yiqi Huoxue Tongmai granule in regulating microglia to mediate cerebral ischemia-reperfusion injury.Methods The BV2 microglia(BV2)model of oxygen and sugar deprivation/reoxygenation was adopted.The experiment objects were divided into five groups for experimental research,including the model group,the blank group,the MMP-9 siRNA group,the Yiqi Huoxue Tongmai granules drug-containing serum intervention group and the MMP-9 siRNA+Yiqi Huoxue Tongmai granules drug-containing serum intervention group.Except blank group,the other four groups were placed in oxygen and sugar deprivation tank for oxygen and sugar deprivation/reoxygenation experiment.Cell proliferation was detected by Cell Counting Kit-8.The expression levels of MMP-9 and TIMP1 in microglia were detected by Western blot.Results The survival rate of microglia cells in the model group was significantly reduced(P<0.01)in comparison with the blank group,and the survival rate of microglia cells in MMP-9 siRNA group,Yiqi Huoxue Tongmai granules drug-containing serum intervention group and MMP-9 siRNA+Yiqi Huoxue Tongmai granules drug-containing serum intervention group was significantly higher(P<0.05 or P<0.01)in comparison with the model group.Compared with blank group,MMP-9 protein expression level of microglia cells in model group was significantly increased(P<0.01),while TIMP1 protein expression level was significantly decreased(P<0.01).The protein expression level of MMP-9 in MMP-9 siRNA group,Yiqi Huoxue Tongmai granules drug-containing serum intervention group and MMP-9 siRNA+Yiqi Huoxue Tongmai granules drug-containing serum intervention group was significantly decreased(P<0.05 or P<0.01),while the protein expression level of TIMP1 was significantly increased(P<0.05 or P<0.01).Conclusion Yiqi Huoxue Tongmai granules can regulate the imbalance of MMP-9/TIMP1 and help to resist microglia cell damage caused by hypoxia and reoxygenation.
Yiqi Huoxue Tongmai GranulesMicrogliaCerebral Ischemia-reperfusionImbalance of MMP-9/TIMP1
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