Objective To compare the therapeutic effects of relaxing needling combined with warming acupuncture in treatment of myofascial pain syndrome(MPS)in the neck and shoulder.Methods 66 cases of neck and shoulder MPS were randomly divided into two groups.The observation group had 31 cases with 2 cases dropped off.The control group had 32 cases with 1 case dropped off.The trigger point(TrP)was the therapeutic target.The observation group was intervened by relaxing needling and warming acupuncture.And the moxibustion was burned two times.The control group was intervened by warming acupuncture.The moxibustion was burned two times.Two groups were intervened once every other day,three times a week for two weeks.Visual analogue Scale(VAS)and cervical dysfunction index(NDI)were evaluated before,immediately,after one week and two weeks after the treatment.Young's modulus and shear wave velocity were evaluated by ultrasound before and after two weeks.Results VAS score and NDI in both groups were lower than before(P<0.01)after treatment Immediately,one week and two weeks.There was no difference between the two groups in VAS score and NDI immediately after treatment and one week after treatment(P>0.05).After 2 weeks of the treatment,the observation group's VAS score and NDI were lower than those in the control group(P<0.05).After 2 weeks of the treatment,the average Young's modulus and average shear wave velocity of the two groups were lower than those before(P<0.01).After 2 weeks of the treatment,the average Young's modulus and average shear wave velocity in the observation group were lower than those in the control group(P<0.05).Conclusion Relaxing needling combined with warming acupuncture and warming acupuncture can relieve pain and improve activity degree of cervical vertebra of patients with neck and shoulder MPS.The effect may be related to the reduce of the TrP's hardness and restore muscle elasticity.With the increase of the treatment times number,the relaxing needling combined with warming acupuncture in the improvement of the pain of patients with neck and shoulder MPS and cervical activity,reducing the organization of the TrP hardness and improving the elasticity of the muscle are better than the warm acupuncture treatment.