Objective To analyze and evaluate the clinical application of indocyanine green fluorescence cholangiography in laparoscopic cholecystectomy due to acute wall-thickening Cholecystitis.Methods A total of 61 patients with acute wall-thickening Cholecystitis were selected,who underwent laparoscopic cholecystectomy in Huzhou Central Hospital during February 2020 to August 2022 and divided into two groups according to the laparoscopic imaging technology.Results Compared with the traditional group,the fluorescence group presented a significant superiority with a shorter time of first-time gallbladder triangle exploration(Z=-6.451,P<0.001),a shorter surgical time(Z=-5.23,P<0.001)and a lower intraoperative blood loss(Z=-4.774,P<0.001).However,there was a little bit advantage of the fluorescence group in bile leakage rate,drainage time and postoperative stay without significant difference.Conclusion Indocyanine green fluorescence cholangiography makes the physicians confident during the laparoscopic cholecystectomy with acute wall-thickening cholecystitis,accelerating the operative progress,shortening the surgical time,lowing the morbidity and enhancing the recovery.Thus,it is worthy of being widely applied in clinic.