Objective To examine the expression of TRIM37 in liver cancer and its influence on the migration of liver cancer cells,as well as to investigate the underlying mechanism of its action.Methods The expression level of TRIM37 mRNA in liver cancer and its correlation with patient survival in the cancer genome atlas database were analyzed using bioinformatics.Additionally,the expression level of TRIM37 protein in liver cancer tissue and adjacent normal tissue was detected through protein immunoblotting(WB).Furthermore,RNA interference technology was employed to apply siRNAs targeting TRIM37 and non-targeted Controls to Huh7 cells,which were divided into a knockdown group(si-TRIM37#1 group,si-TRIM37#2 group)and a non-targeted control group(si-control group).The Transwell migration assay was employed to assess the migration capacity of cells.WB was used to detect the expression of cellular TRIM37 protein and epithelial-mesenchymal transition(EMT)-related marker proteins,as well as the expression of proteins related to PTEN/AKT/GSK-3β/β-catenin signaling pathway.Results Bioinformatics analysis showed that TRIM37 was highly expressed in liver cancer tissue compared to normal tissue adjacent to cancer(P<0.05).Survival analysis showed that the overall survival of patients with high expression of TRIM37 was lower than that of patients with low expression of TRIM37(P<0.05).The Transwell invasion experiment showed that the invasive ability of cells in the si TRIM37 group was significantly lower than that in the si NC group(P<0.05).The WB method showed that the expression levels of N-cadherin and Vimentin proteins in the si-TRIM37 group decreased compared to the si-NC group,while the expression levels of E-cadherin proteins increased,with statistical significance(P<0.05).The si-TRIM37 group exhibited a significant decrease in the expression levels of P-AKT,β-catenin,and P-GSK-3β compared to the si-NC group(P<0.05).Conversely,PTEN protein expression showed a significant increase(P<0.05).Conclusion The expression of the TRIM37 gene is significantly elevated in human liver cancer tissue and exhibits a negative correlation with patient prognosis.Suppression of TRIM37 resulted in the downregulation of EMT marker proteins in liver cancer cells and hindered cell invasion,potentially through the inhibition of the PTEN/AKT/GSK-3β/β-catenin signaling pathway.
万方数据
维普
