Objective To screen the active components of Taohong Siwu Decoction(THSWD)using network pharmacology and predict its mechanism of action on hypertrophic scars(HS).Methods The ingredients and the potential targets of THSWD were predicted with TCMSP,NCBI database and PharmMapper database,and the relevant targets of HS were collected by GeneCards,OMIM,PharmGKB and other databases.The both targets'collections were merged to obtain the ingredients-disease targets.And the protein-protein interaction(PPI)network was prepared with STRING protein database by using Cytoscape 3.8.2 to select the highly focused targets.The DAVID database was applied for GO and KEGG pathway analysis.Immunoblotting assay(Western Blot)was performed to verify the regulatory effects of Tao Hong Si Wu Tang on the selected pathway.Results A total of 425 potential targets of THSWD,1285 HS-related targets were selected,112 of which were highly focused.And the top three targets were ALB,AKT1 and MMP-9.The results of the signaling pathway analysis highly lighted PI3K-Akt signaling pathway,Ras signaling pathway and MAPK signaling pathway et al.The Western blot results showed that Tao Hong Si Wu Tang could regulate the AKT pathway.Conclusion Multiple components in DSO may treat HS by targeting multiple pathways through core targets such as AKT.
关键词
桃红四物汤/网络药理学/增生性瘢痕/AKT信号通路
Key words
Tao hong si wu decoction/Network pharmacology/Hypertrophic scar/AKT signaling pathway
维普
万方数据