首页|显微镜扫描技术与人工镜检在分枝杆菌检测的效果比较

显微镜扫描技术与人工镜检在分枝杆菌检测的效果比较

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目的 评价显微镜扫描技术与传统人工镜检在分枝杆菌检测中的效果差异。方法 选取2021年7月至12月衢州市人民医院就诊的疑似肺结核患者936例,共计痰标本2789份。采用萋-尼(Z-N)抗酸染色,先后进行显微镜扫描系统FS50扫描和人工镜检,以MGIT320快速培养仪的液体培养结果为金标准,比较两种方法的诊断效能及检出时间。结果 FS50和人工镜检阳性检出率分别为8。71%、8。28%,两者差异无统计学意义(P>0。05)。两种方法低阳性级别(实际条数)检出率、平均读片时间比较,差异有统计学意义(P<0。05)。结论 显微镜扫描系统与人工镜检检测分枝杆菌的阳性检出率相当,均有良好的诊断效能,但阳性分级存在差异。扫描系统自动化程度高,可缩短阅片时间,缓解人工镜检的工作强度,在临床检测分枝杆菌方面应用前景良好。
Objective To evaluate the difference in effect of microscope scanning system and traditional manual examination in mycobacteria.Methods The sputum samples from 936 cases(2789)of suspected tuberculosis patients in Quzhou People's Hospital from July 2021 to December 2021 were collected.Zieh I-NeIsen(Z-N)acid resistance staining was performed by automatic tablet dyeing machine,first adding mycobacterial microscope scanning system FS50 for detection,and then manual microscopy of this batch of specimens was carried out,with MGIT320 liquid culture results as the gold standard,and the difference between the diagnostic efficiency and detection time of the two methods were compared.Results The positive detection rates of FS50 and manual microscopy were 8.71%and 8.28%,respectively,and the differences were not statistically significant(P>0.05).Comparison of the detection rate of low positive grade(actual number of strips)and average reading time between the two methods showed statistically significant differences(P<0.05).Conclusion The positive detection rate showed good diagnostic efficacy,but different positive grade.The scanning system has a high degree of automation,shortens the reading time,can greatly alleviate the work intensity of manual microscopy,and has a good application prospect in the clinical detection of mycobacteria.

Microscope scanning systemMycobacteriumManual microscopyZieh-ni acid fast staining

罗梦婷、余旭良、朱扬帆、杨利萍、宋子裕

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310051 浙江中医药大学

324000 温州医科大学附属衢州医院(衢州市人民医院)

325000 温州医科大学

显微镜扫描系统 分枝杆菌 人工镜检 萋-尼抗酸染色

浙江省衢州市科技计划指导性项目

2020047

2024

浙江临床医学
浙江中医药大学 浙江省科普作家协会医学卫生委员会

浙江临床医学

影响因子:0.52
ISSN:1008-7664
年,卷(期):2024.26(8)