Objective To clarify the toxicity of processed Strychnine Semen and Brucine N-oxide,use Strychnine Semen more safely,and further develop and utilize Strychnine Semen and Brucine N-oxide.Methods A total of 210 C57BL/6 mice,half male and half female,which were randomly divided into a control group of 10,administered groups:the Strychnine Semen group,the processed Strychnine Semen group,the Brucine group,the Brucine N-oxide group,50 in each group,were given intragastric administration.The LD50 was determined by the Karber's method.During the period,the general state of the mice,their deaths,and the changes in body weights in the 14-day period after the administration of the four drugs were observed.HE staining was used to observe the pathological tissues damage of hearts,livers,spleens and kidneys in mice that died of poisoning.Results After administration by gavage,the poisoning reactions of the four drugs in mice were similar and showed a dose-dependent aggravation.There was no obvious abnormality in the body weight changes of the mice in each administration group compared with that of the blank control group within 14 days(P＞0.05).The LD50 of processed Strychnine Semen was about 1.6 times of that of Strychnine Semen,the LD50 of Brucine could be up to 31 times of that of Brucine N-oxide.Compared with processed Strychnine Semen and Brucine N-oxide,the death-dose curves of Strychnine Semen and Brucine were steeper and dose-dependent.Compared with the blank control group,HE staining of hearts,livers,spleens and kidneys tissues of mice poisoned and killed in the highest dose group of the four drugs did not show obvious abnormality.Conclusion Processed Strychnine Semen and Brucine N-oxide are less toxic than Strychnine Semen and Brucine,which of treatment window are larger and safer.
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