Objective To explore the impact of serum containing Ferula sinkiangensis from Xinjiang on the apoptosis of HeLa cells by assessing the expression levels of Survivin and Caspase-3 proteins,which are associated with the regulation of apoptosis.Methods Serum containing Ferula sinkiangensis was obtained by administering the substance to rats via gavage,and the serum was then used to treat HeLa cells.The Cell Counting Kit-8(CCK-8)method was used to determine the inhibition rate of cell proliferation.Apoptosis was evaluated using flow cytometry and Hoechst 33258 staining.The expressions of Survivin and Caspase-3 proteins were detected using Western Blot analysis.Results The proliferation inhibitory rates of the drug-containing serum at various concentrations were 11.4％±2.3％,14.9％±3.1％,and 25.3％±3.2％,respectively.The apoptosis rates in the treated groups were 29.4％±9.9％,35.8％±5.3％,and 43.1％±4.7％,respectively.In all treated groups,there was a decrease in Survivin protein and an increase in Caspase-3 protein.As above,these differences were statistically significant compared to the control group(P<0.01).Conclusion Serum containing Ferula sinkiangensis from Xinjiang can promote apoptosis in HeLa cells,which may be related to the suppression of Survivin protein and the up-regulation of Caspase-3 protein expression.
万方数据
维普
