Objective To investigate the correlation between dynamic related protein 1(Drp1)and mitochondrial fission protein 1(Fis1) and clinicopathological features of patients with membranous nephropathy(MN).Methods Retrospective analysis of clinical data of 30 patients with primary membranous nephropathy diagnosed by renal biopsy from January 2017 to December 2018.According to the 24-hour urinary protein quantification,they were divided into a massive proteinuria group(≥3.5 g/d)and a control group(<3.5 g/d).The expression of Drp1 and Fis1 in the renal tissue of the two groups was detected by immunohistochemistry,and the difference between the two groups was compared.And further correlation analysis. Results Drp1 and Fis1 were expressed in podocytes,mesangial cells and proximal tubular epithelial cells.The expression of Drp1 in the massive proteinuria group was significantly higher than that in the control group(P<0.05),and the expression of Drp1 was positively correlated with 24-hour urinary protein quantification(r=0.371,P<0.05).The expression of Fis1 was negatively correlated with age(r=-0.482,P<0.05)and positively correlated with uric acid(r=0.413,P<0.05).Conclusion The expression of Drp1 is positively correlated with proteinuria in MN patients,suggesting that abnormal mitochondrial dynamics may be involved in the pathogenesis of MN proteinuria.Mitochondrial dynamics abnormalities in renal tubular epithelial cells may be associated with early renal tubular injury in MN patients.
万方数据
维普
