Objective To investigate the incidence and influencing factors of complications in preoperative localization of solitary pulmonary nodules using medical glue injection.Methods Totally 79 patients with preoperative medical glue injection localization of pulmonary nodules in our hospital from July 2021 to July 2022 were retrospectively analysed.Patient gender,age,previous lung disease history,basic information of nodules,puncture localization were collected to analyze whether there were differences in the incidence of complications.Logistic regression was used to analyze the independent influencing factors.Results 79 cases of preoperative medical glue injection localization,23 had pneumothorax and 24 had intrapulmonary bleeding. Univariate analysis showed that pneumothorax is ralated to the number of punctures(P<0.05)and intrapulmonary bleeding is ralated to the depth of the lesion,puncture depth,intrapulmonary puncture depth,and colloid diameter(P<0.05).Logistic regression analysis analyzed that the intrapulmonary puncture depth(OR=2.235)and colloid diameter(OR=0.11)were independent influencing factors for intrapulmonary bleeding.Conclutions Preoperative medical glue injection localization is safe and reliable,avoiding repeated puncture,choosing a shorter puncture path,and appropriately increasing the diameter of the glue can effectively reduce complications of preoperative medical glue injection localization.