Exploration on Effect of EA Pretreatment on NCX1 in Cerebral Ischemia Rats Based on'Temporal and Spatial Characteristics'
Objective:To observe the effects of electro-acupuncture(EA)pretreatment on the expression of Na+/Ca2+exchanger 1(NCX1)at different phases in ischemic regions of rats with permanent cerebral ischemia,and to explore its possible mechanism.Methods:84 male SD rats were randomly divided into the sham operation group,the model group and the EA group,and each group was subdivided into subgroups of 0h,1 h,2 h and 24 h,with 7 rats in each one.EA stimulation was applied to Baihui(DU20),bilateral Neiguan(PC6)and Sanyinjiao(SP6),density wave,frequency 2/15 Hz,intensity 1mA,20 min each time for 5 consecutive days.Two hours after the last EA intervention,the rat model of permanent cerebral ischemia was prepared by suture-occluded method.Immunohistochemical staining and Western blot were used to observe the regulation mode of NCX1 positive cells and protein expression in the cortex of different phase(0h,1 h,2 h,24 h)and different ischemic areas(ischemic and non-ischemic areas)by EA pretreatment.Results:In ischemic zone,there was no statistical difference in the expression of NCX1 protein at 0 h among the three groups(P＞0.05);the expressions of NCX1 protein in the model group at 1 h,2 h and 24 h were lower than those of the same time point in the sham operation group(P＜0.05);the expressions of NCX1 protein in the EA group were significantly higher than those in the model group at 1 h and 2 h(P＜0.05),but the difference was not statistically significant compared with that in the model group at 24 h(P＞0.05).In non-ischemic area,there was no statistical difference in the expression of NCX1 protein at 0 h among the three groups(P＞0.05);NCX1 protein expressions were significantly decreased in the model group compared with those in the sham operation group at 2 h and 24 h(P＜0.05);the expression of NCX1 proteins were up-regulated in the EA group than those in the model group at 2 h and 24 h(P＜0.05).Conclusion:The expressions of NCX1 protein in ischemic and non-ischemic cortical areas are decreased in different degrees after cerebral ischemia,EA pretreatment can up-regulate the expressions of NCX1 protein in ischemic zone at 1 h and 2 h,as well as in non-ischemic zone at 2 h and 24 h,which may be one of the ways of its brain protective effect.
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