Mechanism of EA Intervention on'Bone Microecology'in Rats with Ovariectomized Osteoporosis
Objective:To study the effect of electro-acupuncture(EA)on intestinal flora in ovariectomized osteoporosis rats by 16S rRNA sequencing technology,and to explore the mechanism of EA in improving osteoporosis in ovariectomized rats.Methods:SD rats were randomly divided into the sham operation group,the model group,the EA-A group and the EA-B group.The model of ovariectomized osteoporosis was established in rats by removing bilateral ovaries.The rats in the EA-A group and the EA-B group were treated with EA,once a day,and rested for 1 day after 6 days of continuous treatment.The EA-A group was treated for 4 w and the EA-B group was treated for 8 w.Three-point bending test of tibia,vertebral compression test and fecal 16S rRNA sequencing were conducted after the treatment.Results:Compared with those in the sham operation group,the maximum load and the elastic modulus of tibia and vertebrae were decreased significantly in the model group(P＜0.01);compared with those in the model group,the maximum load and the elastic modulus of tibia and vertebra were increased in the EA-A group and the EA-B group after the treatment(P＜0.05,P＜0.01);the improvements were more significant in the EA-B group than those in the EA-A group(P＜0.05,P＜0.01).Firmicutes,Bacteroidetes and Actinobacteria were the top 3 phyla in each group.Compared with those in the sham operation group,the abundance of Firmicutes and Actinobacteria was increased,whereas the abundance of Bacteroidetes was decreased in the model group;for which,there was a different degree callback after EA intervention.According to the results of LEfSe and DESeq2,the dominant flora with significant statistical differences among the groups were analyzed,in the sham operation group the dominant flora were Phascolarctobacterium and Jeotgalicoccus,in the EA-A group were Helicobacter and Oscillospira,and in the EA-B group were Pseudomonas,Dysgonomonas and Janthinobacterium.Beta diversity analysis showed that the abundance of microbiomes was different among the groups.Conclusion:EA may play an anti-osteoporosis role by regulating the abundance and composition of intestinal flora.
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