Jiaji EA Improving Hind Limb Motor Function in Rats With SCI through Inhibiting Xc-/GPX4 Pathway
Objective:To investigate the effect of Jiaji electro-acupuncture(EA)on ferroptosis in rats with spinal cord injury(SCI)and to clarify the action mechanism of EA in promoting SCI repair.Methods:72 rats were randomly divided into the sham operation group,the model group,the Jiaji EA group and the inhibitor(Fer-1)group,with 18 rats in each group;each group was then divided into three subgroups according to different treatment time,including 3 d group,7 d group and 14 d group,with 6 rats in each subgroup.BBB scores and slant plate experiments were performed on 3 d,7 d and 14 d of treatment,followed by HE staining and Nissl's staining,and Western Blot and Elisa assays on spinal cord tissues at the injury stage were applied to observing the expressions of ferroptosis pathway-related factors GSH and GPX4 and the number of neurons.Results:Compared with those in the sham operation group,the BBB scores and the oblique plate angles decreased significantly on 3 d,7 d and 14 d(P＜0.01),and the neurons in the anterior horn of the spinal cord decreased significantly(P＜0.01),and the expressions of GSH and GPX4 decreased significantly in the model group(P＜0.01).Compared with those in the model group,the BBB scores and the oblique plate angles increased significantly on 3 d,7 d and 14 d(P＜0.05),the neurons in the anterior horn of the spinal cord increased significantly(P＜0.01),the expression of GSH increased significantly(P＜0.01),and the expression of GPX4 increased significantly on 7 d and 14 d in the EA group(P＜0.01).Compared with those in the model group,the BBB scores and the oblique plate angles significantly increased on 3 d,7 d and 14 d(P＜0.05),the neurons in the anterior horn of the spinal cord significantly increased(P＜0.01),and the expressions of GSH and GPX4 significantly increased in the inhibitor group(P＜0.01).Compared with those in the inhibitor group,the BBB scores and the oblique plate angles significantly increased on 14 d in the EA group(P＜0.05),but there were no statistical differences in the neurons in the anterior horn of the spinal cord(P＞0.05),also there were no statistical differences in the expressions of GSH and GPX4 between the EA group and the inhibitor group(P＞0.05).Conclusion:Ferroptosis is increased after SCI,and EA treatment can effectively inhibit the Xc-/GPX4 pathway to promote the recovery of motor function of the hind limbs in the rats with SCI.
Jiaji electro-acupunctureFerroptosisSpinal cord injuryMotor function
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