Impact of EA on Repeated Propofol Exposed Inflammatory Response in Rats
Objective:To investigate impact of electro-acupuncture(EA)on propofol-induced inflammatory response.Methods:A total of 40 SD rats(10 d,26 g-28 g)were randomly divided into the control group,the fat emulsion group,the EA group,the propofol group and the combination group,with 8 rats in each group.The control group was given intraperitoneal injection of normal saline(100 μL);the fat emulsion group was given intraperitoneal injection of fat emulsion(100 μL);the EA group was intervened with electro-needling Baihui(DU20)and Dazhui(DU 14),dilatational wave of 2 Hz/10 Hz,30 minutes daily for 3 days;the propofol group was treated with propofol injection(100 mg/kg);the combination group was treated with EA plus propofol injection.Expressions of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were detected by Western blot.The levels of high mobility group protein Bl(HMGB1)and ionized calcium binding adapter molecule 1(Iba-1)in microglia of hippocampus were detected by immunofluorescence.Results:The expressions of TNF-α and IL-6 in the hippocampus were higher(P＜0.05),and the levels of Iba-1 and HMGB1 in the microglia of hippocampus were significantly increased in the propofol group compared with those in the control group(P＜0.05).Compared with those in the propofol group,the expressions of TNF-α and IL-6 were decreased(P＜0.05),and the levels of Iba-1 and HMGB1 were significantly reduced in the combination group(P＜0.05).Conclusion:EA intervention can inhibit the activation and the necroptosis of microglia in the hippocampus,and down-regulate the levels of TNF-α and IL-6,which leads to a reduction in the repeated propofol-induced neuroinflammatory response in rats.
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