摘要
目的:观察电针对2型糖尿病(T2DM)模型大鼠全身炎症及肠黏膜屏障功能的影响.方法:将SD大鼠随机分为正常组、模型组与电针组,每组6只.采用高脂饲料结合单次小剂量链脲佐菌素(STZ)腹腔注射建立T2DM模型.电针组取双侧"足三里""三阴交""脾俞""肾俞",每周3次,给予8周治疗.治疗后检测各组大鼠空腹血糖(FBG);用ELISA法检测大鼠血清中空腹胰岛素(FINS)、肿瘤坏死因子(TNF-α)、白细胞介素-6(IL-6)、内毒素(LPS)、D-乳酸(D-LA)及二胺氧化酶(DAO)含量,并计算胰岛素抵抗指数(HOMA-IR);透射电镜观察大鼠小肠黏膜上皮超微结构;WB检测大鼠小肠上皮紧密连接蛋白ZO-1、Occludin和Claudin-1的表达.结果:与模型组比较,电针能降低T2DM大鼠FBG、FINS和HOMA-IR(P＜0.01),下调血清中炎症因子TNF-α、IL-6含量(P＜0.01),下调血清LPS、D-LA和DAO含量(P＜0.01),上调小肠上皮ZO-1、Occludin及Claudin-1蛋白表达(P＜0.01).结论:电针"足三里""三阴交""脾俞""肾俞"能够降低T2DM大鼠空腹血糖,降低全身炎症并改善胰岛素抵抗状态,其作用机制与上调小肠上皮紧密连接蛋白、改善肠黏膜通透性和修复大鼠肠黏膜屏障功能有关.
Abstract
Objective:To observe the effects of electro-acupuncture on systemic inflammation and intestinal barrier function in rat model of type 2 diabetes mellitus(T2DM).Methods:SD rats were randomly divided into the normal group,the model group and the electro-acupuncture group,with 6 rats in each group.T2DM model was established by high-fat diet combined with single intraperitoneal injection of small-dose streptozotocin(STZ).The electro-acupuncture group was treated with electro-needling Zusanli(ST36),Sanyinjiao(SP6),Pishu(BL20)and Shenshu(BL23),3 times per week for 8 weeks.Fasting blood glucose(FBG)was measured after the treatment in each group.The serum contents of fasting insulin(FINS),tumor necrosis factor(TNF-α),interleukin-6(IL-6),lipopolysaccharide(LPS),D-lactic acid(D-LA)and diamine oxidase(DAO)were detected by ELISA.Homeostasis model assessment of insulin resistance(HOMA-IR)was calculated.Transmission electron microscopy was used to observe the ultrastructure of the small intestinal mucosal epithelium.WB was used to detect the expressions of the small intestinal epithelial tight junction proteins of ZO-1,Occludin and Claudin-1.Results:The levels of FBG,FINS and HOMA-IR were decreased(P＜0.01),the serum inflammatory factors of TNF-α and IL-6 were reduced(P＜0.01),the serum contents of LPS,D-LA and DAO were down-regulated(P＜0.01),and the protein expressions of ZO-1,Occludin and Claudin-1 in the small intestine epithelium were up-regulated in the electro-acupuncture group than those in the model group(P＜0.01).Conclusion:Electro-needling ST36,SP6,BL20 and BL23 can reduce FBG and alleviate systemic inflammation in T2DM rats,which is related to up-regulating small intestinal epithelial tight junction protein,improving intestinal mucosal permeability and repairing intestinal mucosal barrier function.
基金项目
国家自然科学基金(81774420)
肾脏疾病发生与干预湖北省重点实验室项目(SB202108)
针灸治未病科研团队项目(2017zxz004)
维普
万方数据