Effects of Different EA Intervention Time on Cognitive Impairment after Traumatic Brain Injury Based on'Brain-Heart Concurrent Regulation'
Objective:To observe the effects of different electro-acupuncture(EA)intervention time on cognitive impairment after traumatic brain injury(TBI)based on'Brain-Heart concurrent regulation'.Methods:80 male SD rats were randomly divided into the sham operation group,the model group,the EA group 1,the EA group 2,and the EA group 3,with 16 rats in each group.Except for those in the sham operation group,the rats in the rest groups were prepared for the TBI model.EA was administered immediately after modeling in the EA group 1,on the 3 rd day after modeling in the EA group 2 and on the 7 th day after modeling in the EA group 3,and the EA intervention duration was 14 days.The scores of mNSS neurological function,and the performances in novel object recognition test and Morris water maze test were observed.HE staining and FJB staining were used to observe the morphological changes in the tissues around the injury site and the hippocampal region of the affected side.Western blot(WB)and immunofluorescence were used to detect the protein expressions and distributions of IL-1β and IL-18 in the peri-lesion and hippocampus.Results:Compared with those in the model group,the mNSS scores were decreased,the exploration time and preference index for novel objects were increased,the escape latency was shortened,the number of platform crossings in the Morris Water Maze Test was increased,and the expressions and distributions of IL-1β and IL-18 were decreased in the EA groups(P＜0.05).The FJB staining results showed that the number of degenerated neurons in the brains was significantly lower in the EA groups than that in the model group(P＜0.05),and in which there was no statistical difference between the EA group 1 and the sham operation group(P＞0.05).HE staining results showed a more intact morphology of the affected hippocampal region in the EA groups.Among the above indicators,the results in the EA group 1 were better than those in the EA group 2 and the EA group 3.In terms of the indicators in novel object recognition test,Morris water maze test,WB and immunofluorescence detection,there was no statistical differences between the sham operation group and the EA group 1(P＞0.05).Conclusion:The'Brain-Heart concurrent regulation'EA therapy has the effect of improving cognitive function in TBI rats.The earlier the intervention,i.e.,acupuncture immediately after injury,the more significant effect can be achieved in suppressing neuroinflammation,reducing neuronal damage,and improving cognitive impairment after TBI.
Traumatic brain injuryCognitive impairmentBrain-Heart concurrent regulationElectro-acupuncture treatmentIntervention time
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