Effects of EA Intervention on Expressions of ANXA1 and IL-1β in Nucleus Accumbens of Rats with Chronic Sleep Deprivation
Objective:To observe the regulatory effect of electro-acupuncture(EA)on the expressions of pro-inflammatory cytokines annexin A1(ANXA1)and interleukin 1β(IL-1 β)in the nucleus accumbens(NAc)of rats with chronic sleep deprivation(CSD),and to analyze the potential mechanisms of EA in improving sleep and anxiety-like behaviors.Methods:48 male SD rats were randomly divided into the control group,the model group,the EA group,and the sham acupuncture group,with 12 rats in each group.The modified multi-platform method(MMPM)was used to establish the CSD rat model.The EA group was treated with electro-needling Baihui(DU20)and Shenmen(HT7)on the odd-numbered days,and electro-needling Neiguan(PC6)and Taichong(LR3)on the even-numbered days,and the EA intervention duration was 21 days.The general condition and body weight changes were observed.The open field test was conducted to evaluate the spontaneous activity and anxiety levels.The real-time quantitative PCR was used to detect the mRNA expressions of ANXA1 and IL-1 β in the NAc brain region.The Western Blot was used to detect the protein expressions of ANXA1 and IL-1 β in the NAc brain region.Results:After 21 days of modeling,compared with those in the control group,the rats in the rest three groups had dry and dull hair with slow increased weight(P＜0.01);the total distance and the average speed of movement in the open field increased(P＜0.05,P＜0.01),and the time spent in the central area decreased(P＜0.01).After 21 days of intervention,compared with those in the control group,the weight gain was slow(P＜0.01),the total distance and the average speed of movement in the open field were increased(P＜0.01),the time spent in the central area was decreased(P＜0.05),the mRNA and protein expressions of IL-1 β were significantly decreased(P＜0.01),whereas the mRNA and protein expresssions of ANXA1 were increased in the model group at the same period(P＜0.01).Compared with those in the model group,the body weight was gradually increased(P＜0.01),the mental state was good,the total distance and the average speed of movement were decreased(P＜0.05,P＜0.01),the time spent in the central area was increased(P＜0.05),the mRNA and protein expressions of IL-1 β were significantly increased,whereas the mRNA and protein expressions of ANXA1 were significantly decreased in the EA group(P＜0.01).There were no statistical differences between the sham acupuncture group and the model group(P＞0.05).Conclusion:EA can effectively improve sleep and regulate anxiety status of CSD rats,which mechanism may be related to up-regulating IL-1 β and down-regulating ANXA1 in the NAc brain region of CSD rats.
万方数据
维普
