Exploring Mechanism of Xingnao Kaiqiao Needling for Treatment of ICVD Based on Data Mining Technology
Objective:To explore the core acupoints of Xingnao Kaiqiao needling in treatment of ischemic cerebrovascular disease(ICVD),and to predict its mechanism in treating ICVD based on network pharmacology.Methods:The relevant literature of acupuncture treatment for ICVD based on the principle of Xingnao Kaiqiao,which means awakening brain and opening orifices,were retrieved to establish acupuncture prescription by using Excel software.The association rule analysis of acupoint data was carried out to obtain the core points with IBM SPSS Modeler software.The acupoint-target-disease interaction network was visualized through using cytoscape software.String database was used to screen out core targets and build protein-protein interaction(PPI)network.The functional and pathway enrichment analysis of key targets was conducted through DAVID online analysis tool,and the enrichment results were visualized by applying the Bioinformatics free online platform.Results:A total of 70 pieces of literature were included,involving 17 acupoints.Through association rule analysis,the core acupoints of Xingnao Kaiqiao needling included Renzhong(DU26),Neiguan(PC6),Sanyinjiao(SP6),Jiquan(HT1),Chize(LU5)and Weizhong(BL40).According to the acupoint-target-disease network,33 expression factors including TIMP-1,BDNF and VEGF were up-regulated,and 49 expression factors including MMP-9,NSE,IL-6 and TNF-α were down-regulated after core acupoints intervention.Through the PPI network,17 core targets of core acupoints were obtained,including AKT1,IL6,TNF and IL1B.The enrichment analysis showed that the treatment of ICVD by Xingnao Kaiqiao needling may be achieved by working on the lipid and atherosclerosis signaling pathway and the fluid shear force and atherosclerosis signaling pathway.Conclusion:DU26,PC6,SP6,HT1,LU5 and BL40 are the core acupoints for the treatment of ICVD by Xingnao Kaiqiao needling.The core acupoints are involved in the treatment of ICVD by regulating the core targets including AKT1,IL6,TNF,IL1B and other signaling pathways including the lipid and atherosclerosis signaling pathway and the fluid shear force and atherosclerosis signaling pathway.
万方数据
维普
