Effect of Acupuncture Based on Intestine-Brain Communicating Theory on Intestinal Flora and Serum Inflammatory Factors in Epilepsy Model Rats
Objective:To explore the possible mechanism of acupuncture on intestinal flora and inflammation level in epilepsy model rats.Methods:SD rats were randomly divided into the control group,the model group and the acupuncture group,with 10 rats in each group.Epilepsy model was established by intraperitoneal injection of Pentatetrazene(PTZ).The rats in the acupuncture group were treated with needling Baihui(DU20)and Dazhui(DU14),20 min each time;the rats in the control group and the model group were fixed for 20 min each day.The behavior of rats was observed by Morris water maze.The pathological changes of hippocampus were observed by HE staining.The serum levels of IL-6,IL-10 and TNF-α were detected by ELISA.The changes in intestinal flora were observed by 16S rDNA sequencing technique.Results:Compared with those in the control group,the number of rats crossing the platform was decreased(P＜0.05),and the escape latency was significantly extended(P＜0.05),the expressions of IL-6 and TNF-α were decreased significantly(P＜0.05),the expression of IL-10 was increased significantly(P＜0.05),and the relative abundance of intestinal harmfulness was significantly increased in the mode group(P＜0.05).After acupuncture treatment,the number of crossing the platform was significantly increased,and the escape latency was significantly shortened(P＜0.05).The expressions of IL-6 and TNF-α were significantly increased,whereas the expression of IL-10 was significantly decreased.Compared to those in the control group,the relative abundance of intestinal flora of Lactobacillus_reuteri,Prevotella-9,Lactobacillus_murinus,Ruminococcaceae_UCG_014,Lactobacillus_intestinalis,Dubosiella,Ruminococcus_2,Prevotellaceae_NK3B31_group and Akkermansia were significantly increased(P＜0.05);the relative abundance of intestinal flora of Oribacterium,Prevotellaceae_UCG_003,Holdemanella and Lachnospiraceae_UCG-008 were significantly decreased in the model group(P＜0.05).Compared to those in the model group,the relative abundance of g_Rikenellaceae_RC9_gut_group,g_Romboutsia,g_Lachnospiraceae_ND3007_group and g_Quinella and g_Allobaculum were significantly increased in the acupuncture group(P＜0.05).Conclusion:Pentenetetrazon may destroy the homeostasis of intestinal flora,and affect the production of related metabolites and inflammatory factors.Needling DU20 and DU14 can reduce the onset of epilepsy,and the mechanism may be related to the regulation of intestinal flora structure and inhibition of inflammatory response.
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