Study on Neuroprotective Effect of Acupuncture Modulating Shh/Ptch1 Signaling Pathway on Cognitive Impairment in Rat Models of Parkinson's Disease
Objective:To explore whether acupuncture treatment can improve cognitive function in Parkinson's disease(PD)rats by regulating the Shh/Pth1 signaling pathway.Methods:SD rats were randomly divided into the control group,the model group,the acupuncture group,the acupuncture plus Cyclopentamine(Shh inhibitor)group,the Western medication group(Donepezil Hydrochloride),and the acupuncture plus Western medication group.Spatial learning and memory abilities were detected by Morris water maze;the levels of TNF-α,IL-6,IL-10 and IL-1 β in brain tissues were detected by ELISA;the levels of MDA,SOD and CAT in brain tissues were detected by the commercial reagent kit;the morphological changes of hippocampal tissues were observed by HE staining;the protein expressions of Cleaved-caspase-3,Bel-2,Bax,Shh and Ptch1 in hippocampal tissues were detected by Western Blotting.Results:Compared with those in the control group,the neurons showed significant damage;the latency time of the Morris water maze was increased;the levels of TNF-α,IL-6,IL-1β and MDA in hippocampal tissues and protein expressions of Cleaved-caspase-3 and Bax were obviously increased;the residence time and the times of platform crossings were decreased;the level of IL-10,the activities of SOD and CAT,and the protein expressions of Bel-2,Shh and Ptc1 were significantly reduced in the model group(P＜0.05).Compared with those in the model group,the neuronal damage of the rats was significantly reduced;the latency time of Morris water maze was shortened;the levels of TNF-α,IL-6,IL-1 β and MDA in hippocampal tissues were decreased;the protein expressions of Cleaved-caspase-3 and Bax were obviously reduced;the residence time and the times of platform crossings were increased;the level of IL-10,the activities of SOD and CAT,the protein expressions of Bel-2,Shh and Ptc1 were obviously increased in the acupuncture group,the Western medication group,and the acupuncture plus Western medication group(P＜0.05).Compared with those in the acupuncture group,the neuronal damage of the rats was significantly increased;the latency time of Morris water maze was prolonged;the levels of TNF-α,IL-6,IL-1 β and MDA were increased;the protein expressions of Cleaved-caspase-3 and Bax in the hippocampus were significantly increased;the residence time and the times of platform crossings were reduced;the level of IL-10 level,the activities of SOD and CAT,the protein expressions of Bel-2,Shh and Ptch1 were significantly decreased in the acupuncture plus Cyclopamine group(P＜0.05).There were no statistical differences in all detection indicators between the acupuncture group and the Western medication group(P＞0.05).Compared with those in the acupuncture group and the Western medication group,the neuronal damage was significantly reduced;the latency time of Morris water maze was shortened;the levels of TNF-α,IL-6,IL-1 β and MDA were decreased;the protein expressions of Cleaved-caspase-3 and Bax were significantly reduced;the residence time and the times of platform crossing were increased;the level of IL-10,the activities of SOD and CAT,the protein expressions of Bel-2,Shh and Ptch1 were significantly increased in the acupuncture plus Western medication group(P＜0.05).Conclusion:Acupuncture can reduce inflammation and oxidative stress,as well as alleviate neuronal damage in rats with PD cognitive impairment,thereby improve their cognitive function,which possibly through activating the Shh/Ptch1 signaling pathway.
AcupunctureShh/Ptch1 signaling pathwayParkinson's disease cognitive impairmentNeurological function
万方数据
维普
