Study on Mechanism of Mongolian Warm Needling on Sleep Deprivation and Induced Oxidative Stress Response in Rats
Objective:To explore the effect and mechanism of Mongolian warm needling on insomnia and induced oxidative stress response by the experimental study of Mongolian warm needling intervention on sleep deprivation in rats.Methods:The Wistar rats were randomly divided into the blank group,the model group,the Mongolian warm needling group,and the Diazepam group.The sleep deprivation rat model was established through the administration of PCPA via intraperitoneal injection.The alterations in the body weight of rats after sleep deprivation were observed,and the changes in rat nerve cells were observed by electron microscopic.The changes in 5-HIAA,5-HT and NE in brain of rats were observed by fluorescence spectrophotometer.The changes in the contents of SOD and NO in brain stem were observed by grating spectrophotometer.The expressions of CCK-8 and GABA in the brain stem of sleep deprived rats was observed by immunohistochemical staining.Results:The contents of 5-HIAA,5-HT and CCK-8 in reticular formation of the brainstem were decreased,whereas the contents of NE,SOD and NO were increased in the model group(P＜0.01).After sleep deprivation,brain stem cells in the model group of rats exhibited blurred cell membrane boundaries,cloudy cell structures,and vacuolar degeneration.Additionally,the miotchondria showed vacuolar degeneration and significant swelling.Furthermore,the content of rough endoplasmic reticulum within the cells decreased,and synapses fused.However,there were no significant changes in the Mongolian warm needling group.Compared with those in the model group,the contents of 5-HT and 5-HITT were increased,whereas the content of NE was decreased in the Mongolian warm needling group(P＜0.01).The content of NE was significantly decreased in the Diazepam group compared with that in the model group and the Mongolian warm needling group(P＜0.01).Immunohistochemical staining confirmed that after sleep deprivation,the contents of CCK-8 and GABA in reticular formation of the brainstem were significantly increased in the Mongolian warm needling group than those in the model group(P＜0.05,P＜0.01).Conclusion:Mongolian warm needling has a protective effect on nerve cells,and it can restore the coordination and balance between 5-HT and NE pathway,ultimately normalizing the sleep-wake rhythm.It can also alleviate the oxidative stress caused by sleep deprivation,which leads to high energy consumption,increased food intake,and weight loss due to malnutrition.
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维普
