Effect of Acupotomy Intervention on Expressions of p53 Protein and HMGB1 in Premature Aging Chondrocytes of KOA Rabbits
Objective:To observe the effects of acupotomy on p53 protein and HMGB1 in rabbits with knee osteoarthritis(KOA).Methods:24 New Zealand rabbits were randomly divided into the blank group,the model group,the acupotomy group and the electro-acupuncture(EA)group.The KOA model was established by improved Videman method for 6 weeks.The blank group and the model group were not given any intervention,the acupotomy group was treated at the high-stress point around the affected knee joint once a week for 3 weeks.The EA group was treated with electro-needling Xuehai(SP10),Liangqiu(ST34),Neixiyan(EX-LE4)and Waixiyan(EX-LE5),once every other day for 3 weeks,with 20 minutes of needle retention each time.The pathological changes of cartilage morphology of affected side were observed by Safranin O-Fast Green staining method,the contents of interleukin-8(IL-8)and chemokine ligand 2(CCL-2)in joint fluid were detected by ELISA,the expression of HMGB1 was observed by immunohistochemistry,and the expression of p53 protein was observed by Western Blot.Results:Compared with the blank group,the model group showed shallow staining of the cartilage matrix,uneven thickness,disordered arrangement of chondrocytes,and disordered tidal lines;compared with those in the model group,the chondrocyte arrangement was more regular and the tide line was more complete in the acupotomy group and the EA group.Compared with those in the blank group,the levels of IL-8 and CCL-2 in joint fluid,as well as HMGB1 and p53 protein in chondrocytes were higher in the model group(P＜0.01).The levels of IL-8 and CCL-2 in joint fluid,HMGB1 and p53 protein in chondrocytes were lower in the acupotomy group and the EA group than those in the model group(P＜0.05).There were no statistical differences in the levels of IL-8 and CCL-2 in the joint fluid and HMGB1 in chondrocytes between the acupotomy group and the EA group(P＞0.05);however,the expression of p53 protein was less in the acupotomy group than that in the EA group(P＜0.05).Conclusion:Acupotomy intervention can improve the pathological changes of chondrocytes and delay the progression of KOA,which may be related to reducing the expressions of aging related factors HMGB1 and p53.
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