Exploring Related Mechanism of Prefrontal Cortex Involved in EA Therapy for Promoting Consciousness Based on Non-Targeted Metabolomics
Objective:To study the effect of electro-acupuncture(EA)on the metabolites of prefrontal cortex in coma rats with traumatic brain injury(TBI)based on non-targeted metabolomics,and to explore the related mechanism of prefrontal cortex participating in EA therapy for promoting consciousness.Methods:18 SPF healthy SD rats were randomly divided into the blank group,the control group and the EA group,with 6 rats in each group.The rat model of brain injury was prepared by the classic'free fall impact method'.After successful modeling,the EA group was intervened with electro-needling Baihui(DU20)and Shuigou(DU26)for 15 min,and the control group was intervened by acupuncture.The prefrontal cortex tissue was taken,and the protein expressions of y-aminobutyric acid b receptor,aquaporin-4(AQP-4)and malondialdehyde-5(MDA-5)in the prefrontal cortex were detected by Western blot.The metabolites of the prefrontal cortex of each group were analyzed by 1H NMR(1H Nuclear Magnetic Resonance),the differential metabolites were subjected to Kyoto Encyclopedia of Genes and Genomes metabolic pathway enrichment analysis.Results:Compared with those in the blank group,the protein expressions of AQP-4,MDA-5 and GABA b receptor were significantly increased in the control group(P＜0.01).Compared with those in the control group,the protein expressions of MDA-5 and AQP-4 were significantly decreased in the EA group(P＜0.01,P＜0.05);the protein expression of GABA b receptor was decreased,but the difference was not statistically significant.The analysis of differential metabolites showed that a total of 30 endogenous differential metabolites were screened out in the control group compared with those in the blank group.The distribution of the metabolites between the two groups showed obvious dispersion,indicating that the content of each metabolite in the brain tissue was significantly changed after successful modeling.Compared with those in the control group,the outline of metabolites in rat brain tissue was significantly separated in the EA group,indicating that EA intervention had a significant effect on metabolites.Metabolic pathway enrichment analysis showed that the main enrichment pathway of differential metabolites was amino acid metabolism.Conclusion:The molecular mechanism of the involvement of prefrontal cortex in EA therapy for promoting consciousness may be related to the down-regulation of GABA b receptor expression and the effect on the metabolism of excitatory amino acids.
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