首页|黄花杓兰菌根真菌rDNA ITS的多样性

黄花杓兰菌根真菌rDNA ITS的多样性

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  • 国家科技期刊平台
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杓兰属Cypripedium植物因具较高的观赏和药用价值而长期被过度采集,已成为濒危植物.菌根真菌是其栽培和保育能否成功的重要协同因子.采用免培养技术对滇西北4个不同居群的黄花杓兰Cypripedium flavum毛根的真菌进行核糖体脱氧核糖核酸内转录间隔区(rDNA ITS)区段扩增.结果表明:从4个居群的毛根中共克隆得到366个真菌ITS-taxa,其中白水河居群93个,石卡雪山居群90个,天生桥居群103个,纳帕村居群80个;黄花杓兰毛根系统中存在丰富多样的菌根真菌类型,分别涉及胶膜菌属Tulasnella,伏革菌属Corticium,瘤菌根菌属Epulorhiza和丝核菌属Rhizoctoia4个属及一类归属未定的真菌(uncultured mycorrhizal fungi);胶膜菌属对于黄花杓兰具有一定的寄主专一性特征,可能对黄花杓兰的生长有促生作用.以上结果为菌肥的研制和黄花杓兰植物的栽培与保育工作提供科学依据.
The rDNA ITS diversity of mycorrhizal fungi with Cypripedium flavum
Cypripedium plants,which are endangered by over-harvesting due to their high ornamental and medicinal value,use mycorrhizal fungi to guarantee cultivation and conservation.In order to explore the diversity of mycorrhizal fungi,the rDNA internal transcribed spacer (ITS) sequences from mycorrhizal fungi of four C.flavum populations (Baishui River,Shika Snow Mountain,Tiansheng Bridge,and Napa Village) were amplified by nested polymerase chain reaction (PCR) methods.Results showed a total of 336 distinct ITS-taxa of C.flavum obtained from clones of mycorrhizal fungi with populations from Baishui River (93),Shika Snow Mountain (90),Tiansheng Bridge (103),and Napa Village (80).Blast results of rDNA ITS sequences showed a rich diversity in mycorrhizal fungi systems with mycorrhizae belonging to Tulasnella,Corticium,Epulorhiza,and Rhizoctoia.Tulasnella fungi probably had host-specificity and growth-promoting effects on C.flavum.These results may provide a scientific basis for further conservation work of C.flavum and fungal manure development.

microbiologyCypripedium flavummycorrhizal fungirDNA ITSTulasnelladiversity

缪福俊、蒋宏、王宏虬、原晓龙、陈剑、杨宇明、王娟

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云南省林业科学院国家林业局云南珍稀濒特森林植物保护和繁育重点实验室云南省森林植物培育与开发利用重点实验室,云南昆明650201

西南林业大学林学院,云南昆明650224

微生物学 黄花杓兰 菌根真菌 rDNA ITS 胶膜菌属 多样性

云南省应用基础研究计划青年项目国家林业公益性行业科研专项云南省应用基础研究重点项目云南省社会事业发展专项云南省中青年学术技术带头人后备人才培养项目云南藏区典型区域生态安全防控技术研究及应用示范项目云南省教委湿地生态学创新团队项目

2014FD0702012041102013FA0542010CA0102010CI016

2015

10.11833/j.issn.2095-0756.2015.05.024

浙江农林大学学报
浙江农林大学

浙江农林大学学报

CSTPCDCSCD北大核心
影响因子：0.929
ISSN：2095-0756
年,卷(期)：2015.32(5)
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