Cutting-based propagation in Torreya grandis ‘Merrillii’
In Torreya grandis ‘Merrillii’, large-size grafted plantlets cultured for several years are currently used in forestation, while cutting-based propagation has not been used in production. Effects of different sea-sons for cutting, exogenous plant growth regulators and their concentrations, selection and treatment of cuttings, and media on rooting of cuttings were studied in this paper. Results showed that cutting in summer in T. gran-dis ‘Merrillii’ was good using cuttings from less-than-one-year shoots, with a low death rate, a high survival rate and a short rooting time. Semi-woody cuttings treated with NAA at either 100.0 mg·L-1 or 200.0 mg·L-1 had a rooting rate of 64.4% and 55.6%, respectively, which had a higher rooting rate and quality than those treated with IBA and GGR6. Cuttings with a part of 2-year-old shoots treated in summer for 15 s with a 3 721 rooting liquid at a concentration of 2.5 mg·L-1 had a rooting rate of 96.7%, resulting in developed lateral roots and a root system. An optimal medium for hard-wood cutting performed in autumn would be peat∶perlite∶ver-miculite at a proportion of 1 ∶1∶ 1 (V/V/V), which was good in rooting rate and rooting index. The results in-dicated that T. grandis ‘Merrillii’ could get a high rooted plantlet rate by cutting, which had good prospects on direct application to production. But it is necessary to conduct an in-depth study by combining all the re-sults mentioned above for mass production.