为研究蔺草Juncus effusus种质资源遗传多样性,采用简单序列重复区间扩增多态性(inter-simple sequence repeats,ISSR)分子标记技术对来自不同区域的36份蔺草种质资源进行遗传多样性分析,并探讨它们的亲缘关系.结果显示:72对ISSR引物中共筛选出23对引物能扩增出稳定清晰条带;36份蔺草种质的基因组DNA共扩增出148条带,其中130条多态性条带,多态性比率达87.84%.应用NTSYSpc 2.1软件分析显示,36份蔺草种质材料间的遗传相似系数(genetic similarity,GS)为0.4056~0.9441,表明蔺草种质资源具有丰富的遗传多样性.非加权组平均法(unweighted pair group method using arithmetic averages,UPGMA)聚类显示,在遗传相似系数0.6503处可将36份蔺草种质划分为5大类群,第Ⅰ大类群可分为3个亚类,大多数来自于相同或相近地理区域的材料聚于同一类或亚类,野生种质与栽培种质有分别聚在一起的趋势.研究结果从分子水平揭示了蔺草种质资源的主要遗传多态性特点,为今后蔺草育种及杂交亲本的选择提供理论依据.
Genetic diversity of Juncus effusus germplasm by ISSR markers
To study the genetic diversity of Juncus effusus res ources, the genetic diversity of 36 J. effusus germplasm resources from both home and abroad were analyzed by Inter Simple Sequence Repeats (ISSR) markers. Analysis included an Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analy-sis. Results showed 23 primers with clear polymorphic bands selected from 72 primers. A total of 148 bands, of which 130 were polymorphic, were amplified from the 36 materials with the proportion of polymorphic loci be-ing 87.84%. The genetic similarity coefficient for varieties was between 0.4056 and 0.9441, which showed rich genetic diversity. The UPGMA cluster analysis produced a genetic similarity coefficient of 0.6503 for the boundaries with the 36 materials divided into five groups and the first group being divided into three sub-groups. Results were connected to region and cultivar origin with materials from the same region mostly classi-fied into the same group or subgroup. This research revealed the genetic diversity of J. effusus germplasm re-sources at the molecular level for the first time which could provide a theoretical basis for breeding.
国家科技期刊平台
NSTL
万方数据
维普
