Genetic diversity analysis and core collection of pinewood nematodiasis-resistant Pinus massoniana germplasm resources
[Objective]This study,with analyses conducted of the genetic diversity and population structure of 114 germplasm resources of pinewood nematodiasis-resistant Pinus massoniana from Anhui Academy of Forestry and Forestry Technology Extension and Farm tourism Service Center of Linhai in Zhejiang Province,and the construction of a core collection of the germplasm resources,is aimed to provide a theoretical basis for the scientific management and efficient utilization of germplasm resources of P.massoniana.[Method]First,a total of 114 resistant P.massoniana germplasms were detected before their principal co-ordinates analysis(PCoA),population structure and genetic diversity parameters were calculated by bioinformatics software.Then,the core collection was constructed by using M strategy and random sampling strategy so that a comparative analysis was conducted of the genetic diversity indicators of different core collection in order to determine the most suitable construction method.[Result]115 alleles were detected in 114 resistant P.massoniana germplasms,with an average effective allele number(Ne)of 5.54,an average Shannon's diversity index(Ⅰ)of 1.51,and an average PIC value of 0.90 for polymorphic information content,which had a high genetic diversity.The population structure analysis based on Structure software showed that the 114 resistant P.massoniana germplasm resources were divided into four subgroups,and the result of principal coordinate analysis was basically consistent with the above.Based on the genetic diversity parameters and the sampling quantity,the core collection constructed by the M strategy could retain the maximum genetic diversity of the original germplasm with the minimum sampling quantity,which was the optimal sampling strategy.72 core collections were obtained using this strategy,which retained 100％alleles of the original germplasm,with the ration rates of Ne,I,expected heterozygosity(He),PIC being 95.67％,94.96％,98.12％,100.00％.No significant difference in genetic diversity was shown between the constructed core germplasm and the original germplasm according to the PCoA and UPGMA cluster analysis.[Conclusion]The level of genetic diversity of 114 resistant P.massoniana germplasm was high.The 72 core germplasm of resistant P.massoniana were constructed to remove genetic redundancy,which is conducive to the effective conservation and scientific utilization of resistant P.massoniana germplasm resources,and lays the foundation for excellent gene discovery and new cultivar selection.[Ch,7 fig.8 tab.37 ref.]
万方数据
维普
