[Objective]The objective is to study the efficient utilization of nitrogen genes and the ammonium nitrogen transporter protein JrAMT2 gene,which is of great significance for variety improvement,rapid growth,and yield of Juglans regia.[Method]Using J.regia JrAMT2 overexpression(OE)seedlings as experimental materials,bioinformatics analysis was conducted on JrAMT2 gene.The growth and development,nitrogen absorption,chlorophyll content,and chlorophyll fluorescence of J.regia JrAMT2-OE plants were analyzed through gene expression level and phenotype determination.[Result]Fluorescence detection,polymerase chain reaction,and fluorescence quantitative PCR were performed on J.regia JrAMT2-OE plants to verify that the JrAMT2 gene is stably expressed in J.regia JrAMT2-OE plants.Compared with the wild type,the plant height,internode length,biomass and other growth parameters of JrAMT2-OE lines significantly increased,and the plant height and internode length of J.regia seedlings increased by 68.2％and 50.3％.The aboveground and underground biomass of JrAMT2-OE lines increased significantly,with a maximum increase of 56.26％(fresh weight)and 56.26％(dry weight)in the aboveground parts,and a maximum increase of 344.38％(fresh weight)and 354.33％(dry weight)in the underground parts.The uptake of ammonium nitrogen and nitrate nitrogen in the underground part of J.regia JrAMT2-OE significantly increased by 114.1％and 70.3％respectively.The JrAMT2 gene mediated the transport of ammonium nitrogen from the underground part to the aboveground part,and the content of ammonium nitrogen in the aboveground part increased significantly,up to 59.1％.The ratio of chloroplast surface area to monolayer cell surface area and chlorophyll content of JrAMT2-OE increased significantly,with the highest increase of 22.94％and 74.3％.The analysis of chlorophyll fluorescence parameters showed that the activity,quantum yield and electron transfer efficiency of the oxygen-reactive body in the leaves of J.regia JrAMT2-OE plants significantly improved.[Conclusion]The JrAMT2 gene of J.regia plays a significant role in the growth and development,nitrogen absorption and photosynthesis of J.regia seedlings.[Ch,9 fig.2 tab.41 ref.]
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万方数据