Effects of metal ions on digestion and metabolism of Odontotermes formosanus
[Objective]This study aims to explore the role of metal ions in influencing the digestive generation process of Odontotermes formosanus,which is beneficial to explore the potential value of resource treatment of agricultural and forestry waste and kitchen waste containing metal ions by O.formosanus.[Method]According to the concentration gradient method,the bait containing different mass fraction of Al3+,Ca2+,Fe3+and Mg2+was fed to O.formosanus to determine the maximum edible mass fraction of metal ions in bait for O.formosanus.ICP-OES was used to measure the corresponding metal elements in O.formosanus body and new-built fungus combs,and to obtain the effects of feeding baits containing Al3+,Ca2+,Fe3+and Mg2+on the corresponding metal elements mass fraction in O.formosanus body and new-built fungus combs.The activities of laccase and cellulase in O.formosanus body and new-built fungus combs were determined by kit method,and the effects of feeding baits containing Al3+,Ca2+,Fe3+and Mg2+on the activities of these enzymes were determined.[Result]The maximum edible mass fraction of Al3+,Ca2+and Fe3+in baits of O.formosanus was 1.00 g·kg-1,and Mg2+in baits was 10.00 g·kg-1.Mg enrichment occurred both in O.formosanus body and new-built fungus combs after feeding baits containing 10.00 g·kg-1 Mg2+;Fe was only enriched in new-built fungus combs after feeding baits containing 1.00 g·kg-1 Fe3+.The laccase activity in O.formosanus body improved significantly after feeding baits containing 1.00 g·kg-1Al3+.The laccase activity in O.formosanus body improved significantly,but the cellulase activity reduced significantly after feeding baits containing 1.00 g·kg-1 Ca2+.The laccase activity in O.formosanus body and cellulase activity in new-built fungus combs improved significantly,but the laccase activity in new-built fungus combs and cellulase activity in O.formosanus body reduced significantly after feeding baits containing 1.00 g·kg-1 Fe3+.The cellulase activity in O.formosanus body and the laccase activity in new-built fungus combs improved significantly,but the cellulase activity in new-built fungus combs reduced significantly after feeding foods containing 10.00 g·kg-1Mg2+.[Conclusion]O.formosanus can feed on baits containing 1.00 g·kg-1Al3+,Ca2+or Fe3+or 10.00 g·kg-1 Mg2+,and 1.00 g·kg-1 Al3+can improve the combined degradation of lignin by O.formosanus and fungus-comb microbiome.O.formosanus have application potential for resource treatment of agricultural and forestry waste and kitchen waste.[Ch,3 fig.1 tab.30 ref.]
万方数据
维普
