摘要
[目的]探究短时间中波紫外线(UV-B)辐射对三叶青Tetrastigma hemsleyanum酚类成分、抗氧化能力及相关基因表达的影响.[方法]将 3 年生三叶青苗经UV-B持续辐射 12.0 h,在 0、0.5、1.0、3.0、6.0和 12.0 h观察表型;另取三叶青辐射 1.0和 3.0 h(记为T1 和T3)并补充暗处理至 24.0 h(记为T1+23 和T3+21),以未经处理为对照(ck),进行酚类物质(总酚和总黄酮)质量分数、抗氧化能力和叶片中抗逆酶活性、丙二醛(MDA)质量摩尔浓度测定以及转录组分析.[结果]持续UV-B辐射对三叶青表型有影响,3.0 h组叶片局部发黄,6.0和 12.0 h组叶片明显发黄和略微卷曲.叶片和块根的总酚(2.65和 2.63 mg·g-1)和总黄酮(7.16和 7.30 mg·g-1)、块根中的抗氧化能力(86.56%)均在T1+23 组达到最大值.同时,UV-B辐射后施以黑暗处理可促进过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性的大幅提升,且在T1+23 组达到最大,MDA质量摩尔浓度在T3+21 组达到最高(7.21 mmol·g-1).UV-B辐射和黑暗处理下调了黄酮合成通路基因(ThF3H、ThANS、ThCHS、ThANR等)和上调了氧化相关调节因子(CAT和SOD)的表达.[结论]UV-B辐射后增加黑暗处理可提高三叶青酚类物质质量分数、抗氧化能力及抗逆酶活性,且在T1+23 组达最大值,同时下调黄酮合成酶基因表达,上调氧化应激相关基因的表达.图7表2参52
Abstract
[Objective]This study aims to investigate the effects of UV-B radiation on phenolic components,antioxidant capacity and relation genes expression in Tetrastigma hemsleyanum.[Method]Three-year-old T.hemsleyanum seedlings were subjected to continuous UV-B radiation for 12.0 h and their phenotypes were observed at 0,0.5,1.0,3.0,6.0,and 12.0 h.With those untreated as control(ck),some other T.hemsleyanum were irradiated for 1.0 and 3.0 h(T1 and T3),and supplemented with dark treatment for 24.0 h(T1+23 and T3+21).Mass fractions of phenolic substances(total phenols and total flavonoids),antioxidant capacity,anti-stress enzyme activity,malondialdehyde(MDA)mass molar concentration in leaves were determined and transcriptome analysis was made.[Result]Continuous UV-B radiation had an effect on the phenotype of T.hemsleyanum.The leaves of 3-h group were partially yellowed,while the leaves of 6-h and 12-h groups were significantly yellowed and slightly curled.The mass fractions of total phenols(2.65 and 2.63 mg·g-1)and total flavonoids(7.16 and 7.30 mg·g-1)in leaves and root tubers,as well as the antioxidant capacity(86.56%)in root tubers reached the maximum in T1+23 group.Meanwhile,dark treatment after UV-B radiation promoted a significant increase in activities of catalase(CAT),peroxidase(POD)and superoxide dismutase(SOD),which reached the maximum in T1+23 group.The MDA mass molar concentration was the highest in T3+21 group(7.21 mmol·g-1).UV-B radiation and dark treatment down-regulated the expression of flavonoid synthesis pathway genes(ThF3H,ThANS,ThCHS,ThANR,etc.)and up-regulated the expression of oxidation-related regulators(CAT and SOD).[Conclusion]Darkness treatment after UV-B radiation increases the mass fraction of phenolic substances,antioxidant capacity and anti-stress enzymes activity of T.hemsleyanum,reaching the maximum in T1+23 group.The expression of flavonoid synthase genes is down-regulated and the expression of oxidative stress-related genes is up-regulated.[Ch,7 fig.2 tab.52 ref.]
基金项目
浙江省"尖兵领雁"研发攻关计划(2023C02054)
浙江农林大学发展基金(2022LFR111)
国家科技期刊平台
NSTL
万方数据