Identification and analysis of floral scent compounds of Prunus mume cultivars
[Objective]This study,with a comparison of the different floral scent compounds of different cultivars of Prunus mume,is aimed to investigate the floral composition of P.mume so as to lay a foundation for mining the key enzyme genes in the pathway of floral aroma metabolism and molecular breeding in the future.[Method]With 20 cultivars of P.mume from different groups selected as experimental materials,headspace solid phase microextraction method and gas chromatography-mass spectrometry(HS-SPME-GC-MS)were employed to determine the floral components of 20 cultivars of P.mume and identify the characteristic aroma substances of P.mume before an analysis was conducted of the floral components and relative content differences of P.mume in different cultivars groups whereas a cluster analysis was conducted of the cultivars in accordance with the floral components.[Result]A total of 43 volatiles were identified from 20 cultivars of P.mume,among which benzene/phenylpropane compounds were the most diverse and had the highest content,and their relative content in all cultivars was higher than 85%.Among the volatiles,the benzyl acetate,benzyl alcohol,eugenol,methyl eugenol,benzaldehyde and cinnamyl alcohol were the main components of P.mume flower fragrance,the number of floral compounds in Cinnabar Purple and Pink Double group was the highest,followed by that in Versicolor and Green Calyx group,and the number of floral compounds in Alboplena and Pendulous Mei group was relatively smaller.Cluster analysis showed that 20 cultivars of P.mume could be divided into 5 categories according to the types and contents of flower aroma components.[Conclusion]The aroma components and relative contents of different cultivars of P.mume were different,so was the contribution of different floral components to the aroma of different cultivars of P.mume.[Ch,1 fig.11 tab.28 ref.]
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