Identification and expression analysis of bZIP gene family under ABA treatment in Phoebe bournei
[Objective]This study is aimed to identify the bZIP transcription factor family members from Phoebe bournei and investigate the response levels of its members to abscisic acid(ABA)treatment.[Method]A bioinformatic method was employed to identify the PbbZIPs family throughout the whole genome and to analyze its physicochemical properties,gene structure,evolutionary relationships,cis-acting elements in promoter,and the expression patterns under ABA treatment by RT-qPCR.[Result]A total of 63 PbbZIP genes were identified from 12 chromosomes in P.bournei,divided into 12 subfamilies with significantly different in gene structure and motifs,but highly conserved in the same subfamily.Most of the PbbZIPs were localized in the nucleus,and their encoded protein were 110-835 amino acid in length,-1.19--0.19 in hydrophobicity,and 4.48-11.95 in isoelectric point.The 27 pairs of PbbZIPs distributed on 12 chromosomes were featured with collinearity existence,which was the main pattern of PbbZIPs family expansion.A variety of abiotic stress-related action elements were found in the upstream promoter region of PbbZIPs,among which ABA,salicylic acid and methyl jasmonate were more abundant response elements and the genes expression of RT-qPCR revealed that 17 PbbZIPs were induced differentially by ABA signals and generally up-regulated in leaves and roots when P.bournei was treated with 2 mmol·L-1ABA for 1-72 h,with the relative expression of PbbZIPs in roots being generally lower than that in leaves.[Conclusion]The 63 PbbZIPs identified from the P.bournei genome were unevenly distributed across the 12 chromosomes and highly similar in gene sequences,whereas the chromosomal localization genetic structure and conserved motifs were evolutionarily diverse and different between subgroups.The PbbZIPs in leaves and roots responded differently to ABA treatment and got involved in the regulation of other abiotic processes.[Ch,9 fig.35 ref.]
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