Identification and bioinformatics analysis of R2R3-MYB subfamily in cucumber
[Objective]This study is aimed to conduct an in-depth study of the functions associated with members of the R2R3-MYB subfamily in cucumber(Cucumis sativus).[Method]First,an analysis was conducted of the whole cucumber genome so as to identify R2R3-MYB subfamily members.Then,bioinformatics was employed to analyze their phylogenetic relationships,physicochemical properties of proteins,chromosomal localization,gene structure,conserved motifs,and cis-acting elements,as well as the protein interactions.[Result]The whole cucumber genome contained 99 R2R3-MYB transcription factors with typical structural domains,and the protein sequences contained amino acids ranging from 195 to 552,with conserved motifs and amino acid sites.They were unevenly distributed on chromosomes.They were unstable proteins with the instability index of most subfamily members being greater than 40.As was shown by the analysis of cis-acting regulatory elements,most of the elements contained in the promoter regions of genes were closely related to hormone regulation,MYB binding sites,and stress.[Conclusion]The R2R3-MYB family members were obtained through whole cucumber genome identification,which were divided into 30 subgroups and mapped on 7 chromosomes and the upstream promoter region of this family members contained stress-related action elements.[Ch,7 fig.1 tab.36 ref.]
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