Response of soil bacterial community to wetland degradation in the Napahai Plateau
[Objective]This study aims to explore the spatiotemporal dynamic characteristics of soil bacterial structure and diversity in plateau degraded wetlands,so as to provide key data support for understanding the response process and mechanism of soil microbial communities to plateau wetland degradation.[Method]Illumina high-throughput sequencing technology was used to detect the dry and wet seasonal changes of soil bacterial community composition and diversity during different wetland degradation stages(swamp wetlands,swamp meadows,and meadows)in the Napahai Plateau.[Result](1)A total of 10 phyla and 21 genus of soil bacteria were detected,dominated by Proteobacteria and unclassified genera,with relative abundance of 35.92%and 20.64%,respectively.(2)Compared with native swamp wetlands,the relative abundance of Proteobacteria and Acidobacteria in swamp meadows increased by 14.06%and 47.72%,respectively,while that of Chloroflexi significantly decreased by 38.54%(P＜0.05).The relative abundance of Acidobacteria,Actinobacteria,Verrucomicrobia and Planctomycetes in meadows increased by 210.15%,231.37%,229.55%and 315.22%,respectively.The relative abundance of above phyla was greater in the dry season than in the wet season.The relative abundance of Firmicutes in swamp meadows significantly increased by 72.38%in the dry season and decreased by 73.17%in the wet season(P＜0.05).The relative abundance of Bacteroidetes in meadows significantly decreased by 55.50%in the dry season and increased by 223.54%in the wet season(P＜0.05).(3)The Shannon index of swamp meadows and meadows significantly increased compared with native swamp wetlands(P＜0.05).The Shannon indices in swamp wetlands and marsh meadows were higher in the wet season than in the dry season,while those in meadows showed an opposite pattern.(4)Mantel test indicated that the composition of bacterial community structure in the dry season was regulated by soil pH and the concentrations of nitrogen,phosphorus and potassium,while in wet season it was mainly regulated by soil water content,organic matter,nitrogen,phosphorus and pH.The Pearson correlation analysis showed that the controlling factors affecting bacterial diversity in the dry season were soil water content,organic matter,nitrogen and pH,while those in the wet season were soil phosphorus and potassium and carbon nitrogen ratio.[Conclusion]The degradation of plateau wetlands leads to a decrease in soil moisture and pH,which in turn affects the nutrient status of soil carbon,nitrogen,phosphorus,and potassium,ultimately regulating the dry and wet season changes in soil bacterial community composition and diversity.[Ch,4 fig.4 tab.47 ref.]
Napahaiwetland degradationsoil bacteriacommunity diversitydry and wet seasons
国家科技期刊平台
NSTL
万方数据
