Genome-wide identification of the ICE gene family in moso bamboo and its expression pattern under low temperature stress
[Objective]The ICE gene family of Phyllostachys edulis(moso bamboo)was identified and analyzed to find out the key family members responding to cold resistance of moso bamboo,and to lay a theoretical foundation for further research on the biological functions of moso bamboo ICE genes,the molecular mechanisms responding to low-temperature stress,and the genetic transformation to improve the cold resistance of moso bamboo.[Method]Bioinformatics methods were used to identify and analyze the ICE gene family members of moso bamboo,and to analyze the physiological indexes and the expression patterns of ICE genes in moso bamboo at 0,0.5,1.0,24.0,and 48.0 h of low-temperature treatments at 4,0 and-2 ℃.[Result]A total of four mosaic bamboo ICE genes were identified.Conserved structural domains and multiple sequence comparison analyses showed that their structures were highly similar.Phylogenetic relationships and promoter cis-acting element analyses showed that the PeICE gene was more closely related to rice,and a large number of cis-acting elements associated with abiotic stresses were also present.Reactive oxygen radical(superoxide anion)staining revealed that the ROS staining gradually deepened with the increase in treatment time,but its staining gradually weakened after 24.0 h of 0 ℃ treatment and 1.0 h of-2 ℃ treatment.Proline content and superoxide dismutase activity assays revealed an overall increase in Pro and SOD content at 4 ℃ and 0 ℃,but their expression was lower than the control at-2 ℃,and peroxidase activity assays revealed an increase at all three temperatures.Analysis of ICE gene expression pattern revealed that the overall expression of PeICE increased in 4 ℃ and 0 ℃ treatment,and the most obvious increase was in PeICE3.The overall expression level of PeICE was lower than that of the control at-2 ℃.[Conclusion]With the decrease of cold treatment temperature and the enhancement of treatment time,moso bamboo was subjected to increasing damage,and its in vivo enzyme activity system as well as ICE genes responded positively to cold stress,and PeICE3 was the most sensitive to cold stress,whereas the expression of ICE genes did not increase at-2 ℃,and this gene family maybe respond to low-temperature stress instead of freezing stress.[Ch,7 fig.1 tab.42 ref.]
Phyllostachys edulislow temperature stressICE gene familygene identificationexpression analysis
万方数据
维普
