Genetic characteristics of whole chloroplast genome in Prunus triloba'Multiplex'
[Objective]To investigate the phylogenetic location of Prunus triloba'Multiplex'and analyze its chloroplast gene composition characteristics,the whole chloroplast genome sequence of P.triloba'Multiplex'was sequenced.[Method]The leaves of P.triloba'Multiplex'were used as materials to extract chloroplast DNA using 2×CTAB method.The whole chloroplast genome was sequenced by Illumina NovaSeq platform.We then assembled,annotated,and analyzed the genetic characteristics of the chloroplast genomic sequence.Combined with the NCBI database data,the phylogenetic relationship of P.triloba'Multiplex'was constructed based on the whole chloroplast genome sequence.[Result]The total length of the chloroplast genome of P.triloba'Multiplex'is 157 827 bp,with NCBI registration number MT937181.The structure is a classic tetrad structure,consisting of 1 LSC(large single copy region),1 SSC(small single copy region),and IRa/IRb(reverse repeat region),with sequence lengths of 86 032,19 023 and 26 386 bp,respectively.The total content of GC and AT is 36.80％and 63.20％,respectively.The complete chloroplast genome sequence of P.triloba'Multiplex'was annotated to 132 genes,including 37 tRNA genes,87 coding protein genes,and 8 rRNA genes,respectively.The chloroplast genome of P.triloba'Multiplex'encodes a total of 26 678 codons and 236 eligible SSR loci.The A/T base is dominant in the SSR site,and the base preference is very obvious.[Conclusion]The phylogenetic tree analysis showed that P.triloba'Multiplex'and P.triloba aggregate into a branch structure,and are closely related to P.pedunculata in the same genus.The results of this study will provide new evidence and basic experimental data for species identification,phylogenetic evolution,and breeding and selection of P.triloba'Multiplex'.[Ch,4 fig.4 tab.26 ref.]
万方数据
维普
