Cloning and function analysis of LsMYB7 gene in Lycoris sprengeri
[Objective]The purpose is to study the regulatory effect of LsMYB7 gene on the accumulation of anthocyanins in Lycoris sprengeri.[Method]R2R3-MYB transcription factor LsMYB7 was screened from petals of L.sprengeri.The expression of LsMYB7 and structure genes related to anthocyanin biosynthesis was analyzed by real-time quantitative PCR technology(RT-qPCR).The function of LsMYB7 on anthocyanin accumulation was studied by virus-induced gene silencing(VIGS)technology.[Result]A 951 bp LsMYB7 gene cDNA sequence was cloned from petals in L.srprengei,containing 825 bp open reading frame(ORF)which encoded 274 amino acids.LsMYB7 protein contained two R2 and R3 domain,and belonged to R2R3-MYB transcription factor family.Phylogenetic analysis showed that LsMYB7 was clustered one group with Arabidopsis thaliana S22 subfamily genes,involved in regulating responses to abiotic stress such as drought and high temperature.LsMYB7 was localized in the nucleus.The expression trend of LsMYB7 gene was consistent with genes related to anthocyanin biosynthesis in different flower development stages and different flower color clones,and mainly expressed in petals of Hl clones with high anthocyanin content and last flowering period.The petals of L.sprengeri became significantly shorter and darker in color,and the expression of genes related to anthocyanin biosynthesis,such as LsCHS,LsF3'H,LsANS,LsUFGT1,and LsUFGT2,was significantly downregulated after LsMYB7 gene being silenced.[Conclusion]LsMYB7 belongs to S22 subfamily of the R2R3-MYB transcription factor family,and gets involved in regulating the expression of genes(like LsCHS,LsF3'H,LsANS,LsUFGT1 and LsUFGT2)related to anthocyanin biosynthesis and promoting anthocyanin accumulation.[Ch,9 fig.1 tab.31 ref.]
Lycoris sprengeriR2R3-MYB transcription factoranthocyanin accumulationvirus-induced gene silencing(VIGS)regulation function
万方数据
维普
