Cutting propagation of softwood and dynamic changes in physiological indicators during the rooting process of Cornus hongdongensis subsp.tonkinensis
[Objective]This study,with an investigation of the effects of plant growth regulator soaking,substrate type and growth stage of cutting scion on rooting of Cornus hongdongensis subsp.tonkinensis as well as the morphological characteristics and physiological and biochemical properties of cutting during the rooting process,is aimed to establish a scientific basis for its propagation and production.[Method]First,using clear water treatment as a control,an orthogonal design was performed for plant growth regulator treatment,including types(ABT-1,NAA and IAA),concentrations(100,300 and 500 mg·L-1)and soaking duration(30,60 and 180 min);then,4 types of cutting substrates were prepared,containing vermiculite(SI),river sand(S2),V(peat soil)∶V(vermiculite)∶V(perlite)=2∶2∶1(S3)and V(peat soil)∶V(loess)=1∶1(S4);afterwards,cutting scions were collected at three stages on July 12,August 15 and September 1,respectively before the rooting index was statistically analyzed,and the changes of nutrient content and rooting-related enzyme activities in the process of rooting were measured.[Result](1)The rooting type was dominated by skin rooting,which accounted for 58.60％of the total number of roots.(2)In the orthogonal test,the hightest rooting rate of 77.78％was achieved by soaking the cutting with 300 mg·L-1 IAA for 30 min.(3)In the cutting substrate test,the highest rooting rate and rooting index were found in S3 substrate treatment(82.22％and 19.34).(4)In growth stage test,mid-growth cutting rooting was highest(82.88％).(5)Compared with the control treatment,the IAA treatment effectively promoted nutrient consumption in the cutting and increased the activity of oxidative enzymes.(6)Correlation analysis showed that rooting rate was significantly negatively correlated with soluble sugars,starch and soluble proteins during the period of root primordium induction and healing formation(P＜0.05),and highly significantly positively correlated with POD and SOD(P＜0.01).[Conclusion]Cutting scion taken at mid-growth through 300 mg·L-1 IAA for 30 min treatment and embed in S3 substrate achieved the highest rooting percentage and rooting index up to 82.22％and 19.34,respectively.[Ch,2 fig.7 tab.30 ref.]
万方数据
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