[目的]探究浙江省杂草稻Oryza sativa f spontanea种子对不同胁迫环境的耐受性，为稻种遗传改良和杂草稻防控提供依据。[方法]以浙江省杂草稻和同一田块采集的栽培稻'南粳46'O。sativa'Nangeng 46'种子为材料，分别在室内标准发芽条件、盐胁迫(0。15 mol·L-1 NaCl)、干旱胁迫(质量分数为15％的聚乙二醇溶液模拟)、淹水胁迫(5 cm水深模拟)和低温胁迫(15 ℃低温)下研究种子的发芽情况和幼苗品质，并测定了干旱和盐胁迫下幼苗的抗氧化物酶活性和丙二醛质量摩尔浓度。[结果]室内标准发芽条件下，杂草稻种子的各项发芽指标均显著优于栽培稻(P＜0。05)。不同环境胁迫均抑制了种子萌发，影响了幼苗品质。盐胁迫下，杂草稻种子的发芽率和发芽指数显著高于栽培稻，幼苗长度低于栽培稻(P＜0。05)。干旱胁迫下，杂草稻种子的发芽势、发芽率和发芽指数均显著高于栽培稻(P＜0。05)。干旱和盐胁迫后，杂草稻幼苗的过氧化氢酶、超氧化物歧化酶和过氧化物酶活性显著高于栽培稻，丙二醛质量摩尔浓度显著低于栽培稻(P＜0。05)。淹水胁迫下，杂草稻的发芽势、发芽指数均显著低于栽培稻(P＜0。05)。低温胁迫后，杂草稻和栽培稻种子的发芽指标无显著差异，杂草稻幼苗质量较栽培稻更好。[结论]浙江省杂草稻在适宜环境下的萌发能力显著强于栽培稻，但对淹水胁迫敏感。与栽培稻相比，杂草稻更耐干旱和盐胁迫，这种能力可能与其具备更强的抗氧化活性有关。表6参32
Identification of seed germination ability of weedy rice under stress in Zhejiang Province
[Objective]The aim is to investigate the tolerance of weedy rice(Oryza sativa f.spontanea)seeds to different stress environments in Zhejiang Province,and to provide evidence for genetic improvement of rice varieties and prevention and control of weedy rice.[Method]The seeds of weedy rice and cultivated rice(O.sativa'Nangeng 46')collected from the same field in Zhejiang Province were used as material.The germination of seeds and seedling quality were studied under indoor standard germination condition,salt stress(0.15 mol·L-1 NaCl),drought stress(simulated with 15％PEG 6000),flooding stress(simulated with 5 cm water depth),and low temperature stress(at 15 ℃).The antioxidant enzyme activities and malondialdehyde(MDA)content of seedlings under drought and salt stress were measured.[Result]Under standard germination conditions,the germination indicators of weedy rice seeds were significantly better than those of cultivated rice(P＜0.05).Different environmental stresses inhibited seed germination and affected seedling quality.Under salt stress,the germination rate and germination index of weedy rice seeds were significantly higher than those of cultivated rice,and the seedling length was lower than that of cultivated rice(P＜0.05).Under drought stress,the germination potential,germination rate,and germination index of weedy rice seeds were significantly higher than those of cultivated rice(P＜0.05).After drought and salt stress treatment,the activities of catalase,superoxide dismutase,and peroxidase in weedy rice seedlings were significantly higher than those in cultivated rice,and the MDA content was significantly lower than that in cultivated rice(P＜0.05).Under flood stress,the germination potential and germination index of weedy rice were significantly lower than those of cultivated rice(P＜0.05).Under chilling stress,there was no significant difference in germination indicators between weedy rice and cultivated rice seeds,and the quality of weedy rice seedlings was better than that of cultivated rice.[Conclusion]The germination ability of weedy rice in Zhejiang Province is significantly stronger in suitable environments than that of cultivated rice in the same region,but it is sensitive to flooding stress.Compared with cultivated rice,weedy rice is more tolerant to drought and salt stress,which may be related to its stronger antioxidant activity.[Ch,6 tab.32 ref.]
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