Cloning and functional identification of SmJRB2 gene in Salvia miltiorrhiza
[Objective]Salvia miltiorrhiza is a traditional Chinese medicine used in clinical treatment of cardiovascular and cerebrovascular diseases.Elucidating the molecular regulation mechanism of metabolism and synthesis of pharmacophore of S.miltiorrhiza can provide scientific basis for breeding new varieties of S.miltiorrhiza with high quality.[Method]The transcriptional factor SmJRB2 in response to methyljasmonic acid(MeJA)induction was picked out based on comparative transcriptome mining.The coding sequence of this gene was cloned using homologous cloning technology and analyzed by bioinformatics.The tissue expression and MeJA induced expression of SmJRB2 gene were detected by quantitative real-time polymerase chain reaction(RT-qPCR).The function of SmJRB2 gene was identified based on the genetic transformation technology of S.miltiorrhiza mediated by Agrobacterium tumefaciens.[Result]The results showed that SmJRB2 encoded 501 amino acids and belonged to the MYC transcription factor of bHLH transcription factor family.The expression of SmJRB2 gene was the highest in leaves and principal root.SmJRB2 gene was intensively induced by MeJA and its highest expression level peaked at the induction time of 4.0 h.Overexpression of SmJRB2 promoted the accumulation of tanshinones and suppression of SmJRB2 gene decreased the biosynthesis of tanshinones.[Conclusion]SmJRB2 is a positive regulator of tanshinone metabolic synthesis.[Ch,8 fig.1 tab.40 ref.]
Salvia miltiorrhizaSmJRB2cloningexpression profilefunctional identification
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