Comprehensive quality evaluation of Saposhnikoviae radix based on multi-component contents determination
[Objective]To establish a method for quantitative analysis of multi-index components in Saposhnikoviae radix(Saposhnikovia divaricata),and for comprehensive quality evaluation of the medicinal materials from different areas,using the contents of multiple-index components as evaluation indexes,and the contribution of different components to the anti-inflammatory activity.[Method]The concentrations of 6 chromones and 4 coumarins from Saposhnikoviae radix were quantified by high-performance liquid chromatography(HPLC).The quality was comprehensively evaluated using cluster analysis and principal component analysis.The correlations between 10 chemical components and their anti-inflammatory activities were analyzed in a lipopolysaccharide-induced macrophage inflammation model in RAW 264.7 mice.[Result]The 10 kinds of chemical components had good linear relationship,accuracy,and separation in their respective ranges,respectively.17 batches of samples were grouped into 4 categories by cluster analysis and principal component analysis.The quality of 2-year-old S.divaricata grown in Inner Mongolia(S5)and Jilin(S8 to S11)were relatively better.17 batches of SS.divaricata alcohol extracts differentially inhibited NO,IL-6,and IL-1βsecreted by lipopolysaccharide-induced RAW 264.7 cells.Prim-O-glucosylcimifugin,5-O-methylvisammioside,cimifugin,sec-O-glucosylhamaudol,5-O-methylvisamminol,and imperatorin were positively correlated with anti-inflammation.The anti-inflammatory effect of S.divaricata is the result of the combined action of several components,and the correlation degrees among prim-O-glucosylcimifugin,5-O-methylvisammioside,cimifugin,sec-O-glucosylhamaudol,and 5-O-methylvisamminol with three cellular indicators of inflammation(NO,IL-6,and IL-1 β)were over 0.8,which were the main material basis for the anti-inflammatory activity,according to the gray correlation analysis.[Conclusion]A comprehensive evaluation model of the quality of S.divaricata based on biological activity and combining the anti-inflammatory activity and gray correlation analysis was established,which provided a new method and scientific basis for the establishment of quality standards for a comprehensive evaluation of the quality of SS.divaricata.[Ch,3 fig.7 tab.30 ref.]
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