暗影饰皮夜蛾线粒体基因组全序列测定与分析
Sequencing and analysis of the complete mitochondrial genome of Garella ruficirra
李妍 1舒金平 1华克达 2张亚波 1应玥 1张威1
作者信息
- 1. 中国林业科学研究院亚热带林业研究所,浙江杭州 311400
- 2. 杭州市富阳区农业农村局,浙江杭州 311400
- 折叠
摘要
[目的]对薄壳山核桃Carya illinoensis害虫暗影饰皮夜蛾Garella ruficirra线粒体基因组进行测序和分析,并在基因组水平上探讨其在夜蛾科Noctuidae中的分类地位,为探索夜蛾科昆虫的系统发育关系以及演化进程提供参考.[方法]利用二代测序技术从头组装获取暗影饰皮夜蛾的线粒体基因组,并对线粒体基因组结构特点和碱基组成进行分析;同时,采用最大似然法和贝叶斯法联合构建了夜蛾科5个属、12个种的线粒体基因组系统发育树,分析暗影饰皮夜蛾在夜蛾科中的系统发育地位.[结果]暗影饰皮夜蛾线粒体基因组全长共为15 294 bp,其中包括13个蛋白质编码基因、22个转运RNA基因、2个核糖体RNA基因以及鳞翅目Lepidoptera昆虫典型的腺嘌呤(A)+胸腺嘧啶(T),即A+T富含区,该区域的A+T含量为80.53％,具有明显的AT偏向性.暗影饰皮夜蛾的基因排列顺序为trnM-trnI-trnQ,与包括夜蛾科昆虫在内的大多数鳞翅目昆虫基因排列次序相符.13个蛋白质编码基因的起始密码子全部为ATN.22个tRNA基因中除trnS1的DHU臂缺失,其余均为典型的三叶草结构.对线粒体基因组研究发现:夜蛾科5个属之间,Garella与皮夜蛾属Nycteola亲缘关系最近,与饰夜蛾属Pseudoips亲缘关系最远.[结论]暗影饰皮夜蛾的线粒体基因组中出现了基因重排的现象,系统发育关系支持暗影饰皮夜蛾和Garella musculana聚为1个分支.图4表4参47
Abstract
[Objective]This study,with a comprehensive sequencing and analysis of the mitochondrial genome of Garella ruficirra as well as an investigation into the phylogenetic position of Noctuidae through mitochondrial genome sequencing,is aimed to offer a valuable reference for delving into the phylogenetic relationships and evolutionary processes within the Noctuidae as well as exploring the phylogenetic relationship and evolutionary process of Noctuidae.[Method]First,the Illumina sequencing technique was employed to sequence the complete mitochondrial genome of G.ruficirra,allowing for the analysis of its overall characteristics and base composition.Subsequently,a phylogenetic tree encompassing mitochondrial genomes from 5 genera of Noctuidae,comprising 12 species,was constructed using both maximum likelihood and Bayesian methods so as to facilitate the examination of the phylogenetic relationships within the Noctuidae.[Result]The circular mitochondrial genome of G.ruficirra spanned 15 294 base pairs and encompasses 13 protein synthesis-related genes,22 transfer RNA genes,and two ribosomal RNA genes and its gene arrangement conformed to the standard configuration observed in Lepidoptera mitogenomes,featuring a region rich in adenine and thymine,an A+T content of 80.53％and a significant AT bias.The gene order of trnM-trnI-trnQ in G.ruficirra aligned with other Noctuidae species and the majority of Lepidoptera with ATN sequences serving as initiation codons for all 13 protein-coding genes.While trnSl lacked a DHU arm,the remaining tRNA genes exhibited a typical cloverleaf-shaped structure.According to the study of mitochondrial genome,Garella was most closely related to Nycteola and least closely related to Pseudoips among the 5 genera.[Conclusion]Gene rearrangement was evident in the mitochondrial genome of G.ruficirra.Phylogenetic analyses,indicating that the clustering of G.ruficirra and G.musculana samples was within a single clade.This study has furnished valuable insights into the evolutionary patterns of Garella and offered a foundational framework for investigating the phylogeny and evolution of Noctuidae within the Lepidoptera order.[Ch,4 fig.4 tab.47 ref.]
关键词
暗影饰皮夜蛾/线粒体基因组/系统发育关系/基因重排Key words
Garella ruficirra/mitochondrial genome/phylogenetic relationship/gene rearrangement引用本文复制引用
基金项目
中国林业科学研究院基本科研业务费专项(CAFYBB2022SY011)
出版年
2024
国家科技期刊平台
NSTL
万方数据